Improved DNA-based identification of cervidae species in forensic investigations
2017
Аутори
Davitkov, DajanaGlavinić, Uroš
Nešić, Ksenija
Davitkov, Darko
Vučićević, Miloš
Nešić, Vladimir
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The main reasons for wildlife forensic research are animal poaching, illegal trade, and falsified game meat products. Small trace amounts, old and degraded materials present the most common samples in revealing criminal activities in this field. This is the reason why it is crucial to use adequate and reliable methods and samples to identify animal species killed outside the hunting season or species protected by law. In this study, different endpoint PCR and real-time PCR protocols were compared in the identification of three Cervidae species (Capreolus capreolus, Cervus elaphus, Dama dama) from old and damaged material found in an enclosed area where the animals were kept. From a total of 129 samples, end point PCR provided results for 119 samples, while real-time PCR was successful in all cases. Also, we created and tested a protocol for simultaneous analyses of different types of samples, which is of great importance as when the amplification is carried out simultaneously it is mor...e cost efficient and speeds up the process.
Кључне речи:
Cervidae species / forensics / molecular analysis / poachingИзвор:
Acta Veterinaria-Beograd, 2017, 67, 4, 449-458Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
DOI: 10.1515/acve-2017-0037
ISSN: 0567-8315
WoS: 000419653200001
Scopus: 2-s2.0-85041201981
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Davitkov, Dajana AU - Glavinić, Uroš AU - Nešić, Ksenija AU - Davitkov, Darko AU - Vučićević, Miloš AU - Nešić, Vladimir AU - Stanimirović, Zoran PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1521 AB - The main reasons for wildlife forensic research are animal poaching, illegal trade, and falsified game meat products. Small trace amounts, old and degraded materials present the most common samples in revealing criminal activities in this field. This is the reason why it is crucial to use adequate and reliable methods and samples to identify animal species killed outside the hunting season or species protected by law. In this study, different endpoint PCR and real-time PCR protocols were compared in the identification of three Cervidae species (Capreolus capreolus, Cervus elaphus, Dama dama) from old and damaged material found in an enclosed area where the animals were kept. From a total of 129 samples, end point PCR provided results for 119 samples, while real-time PCR was successful in all cases. Also, we created and tested a protocol for simultaneous analyses of different types of samples, which is of great importance as when the amplification is carried out simultaneously it is more cost efficient and speeds up the process. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Improved DNA-based identification of cervidae species in forensic investigations VL - 67 IS - 4 SP - 449 EP - 458 DO - 10.1515/acve-2017-0037 ER -
@article{ author = "Davitkov, Dajana and Glavinić, Uroš and Nešić, Ksenija and Davitkov, Darko and Vučićević, Miloš and Nešić, Vladimir and Stanimirović, Zoran", year = "2017", abstract = "The main reasons for wildlife forensic research are animal poaching, illegal trade, and falsified game meat products. Small trace amounts, old and degraded materials present the most common samples in revealing criminal activities in this field. This is the reason why it is crucial to use adequate and reliable methods and samples to identify animal species killed outside the hunting season or species protected by law. In this study, different endpoint PCR and real-time PCR protocols were compared in the identification of three Cervidae species (Capreolus capreolus, Cervus elaphus, Dama dama) from old and damaged material found in an enclosed area where the animals were kept. From a total of 129 samples, end point PCR provided results for 119 samples, while real-time PCR was successful in all cases. Also, we created and tested a protocol for simultaneous analyses of different types of samples, which is of great importance as when the amplification is carried out simultaneously it is more cost efficient and speeds up the process.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Improved DNA-based identification of cervidae species in forensic investigations", volume = "67", number = "4", pages = "449-458", doi = "10.1515/acve-2017-0037" }
Davitkov, D., Glavinić, U., Nešić, K., Davitkov, D., Vučićević, M., Nešić, V.,& Stanimirović, Z.. (2017). Improved DNA-based identification of cervidae species in forensic investigations. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 449-458. https://doi.org/10.1515/acve-2017-0037
Davitkov D, Glavinić U, Nešić K, Davitkov D, Vučićević M, Nešić V, Stanimirović Z. Improved DNA-based identification of cervidae species in forensic investigations. in Acta Veterinaria-Beograd. 2017;67(4):449-458. doi:10.1515/acve-2017-0037 .
Davitkov, Dajana, Glavinić, Uroš, Nešić, Ksenija, Davitkov, Darko, Vučićević, Miloš, Nešić, Vladimir, Stanimirović, Zoran, "Improved DNA-based identification of cervidae species in forensic investigations" in Acta Veterinaria-Beograd, 67, no. 4 (2017):449-458, https://doi.org/10.1515/acve-2017-0037 . .