Isolation and detection of Listeria monocytogenes in poultry meat by standard culture methods and PCR
2017
Authors
Kureljušić, JasnaRokvić, Nikola I.
Jezdimirović, Nemanja
Kureljušić, Branislav
Pisinov, Boris
Karabasil, Nedjeljko
Conference object (Published version)
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Listeria is the genus of a bacteria found in soil and water and some animals, including poultry and cattle. It can be present in raw milk and food made from raw milk. It can also live in food processing plants and contaminate a variety of processed meats. Microscopically, Listeria species appear as small, Gram-positive rods, which are sometimes arranged in short chains. In direct smears, they can be coccoid, so they can be mistaken for streptococci. Longer cells can resemble corynebacteria. Flagella are produced at room temperature but not at 37 degrees C. Haemolytic activity on blood agar has been used as a marker to distinguish Listeria monocytogenes among other Listeria species, but it is not an absolutely definitive criterion. Further biochemical characterization is necessary to distinguish between the different Listeria species. The objective of this study was to detect, isolate and identify Listeria monocytogenes from poultry meat. Within a period of six months from January to Ju...ne 2017, a total of 15 samples were collected. Three samples were positive for the presence of Listeria monocytogenes. Biochemical and microbiological tests as well as PCR technique using specific primers were used to confirm L. Monocytogenes in the samples.
Source:
59th International Meat Industry Conference (MEATCON2017), 2017, 85, UNSP 012069-Publisher:
- Iop Publishing Ltd, Bristol
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
DOI: 10.1088/1755-1315/85/1/012069
ISSN: 1755-1307
WoS: 000418556900069
Scopus: 2-s2.0-85030623010
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Fakultet veterinarske medicineTY - CONF AU - Kureljušić, Jasna AU - Rokvić, Nikola I. AU - Jezdimirović, Nemanja AU - Kureljušić, Branislav AU - Pisinov, Boris AU - Karabasil, Nedjeljko PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1523 AB - Listeria is the genus of a bacteria found in soil and water and some animals, including poultry and cattle. It can be present in raw milk and food made from raw milk. It can also live in food processing plants and contaminate a variety of processed meats. Microscopically, Listeria species appear as small, Gram-positive rods, which are sometimes arranged in short chains. In direct smears, they can be coccoid, so they can be mistaken for streptococci. Longer cells can resemble corynebacteria. Flagella are produced at room temperature but not at 37 degrees C. Haemolytic activity on blood agar has been used as a marker to distinguish Listeria monocytogenes among other Listeria species, but it is not an absolutely definitive criterion. Further biochemical characterization is necessary to distinguish between the different Listeria species. The objective of this study was to detect, isolate and identify Listeria monocytogenes from poultry meat. Within a period of six months from January to June 2017, a total of 15 samples were collected. Three samples were positive for the presence of Listeria monocytogenes. Biochemical and microbiological tests as well as PCR technique using specific primers were used to confirm L. Monocytogenes in the samples. PB - Iop Publishing Ltd, Bristol C3 - 59th International Meat Industry Conference (MEATCON2017) T1 - Isolation and detection of Listeria monocytogenes in poultry meat by standard culture methods and PCR VL - 85 SP - UNSP 012069 DO - 10.1088/1755-1315/85/1/012069 ER -
@conference{ author = "Kureljušić, Jasna and Rokvić, Nikola I. and Jezdimirović, Nemanja and Kureljušić, Branislav and Pisinov, Boris and Karabasil, Nedjeljko", year = "2017", abstract = "Listeria is the genus of a bacteria found in soil and water and some animals, including poultry and cattle. It can be present in raw milk and food made from raw milk. It can also live in food processing plants and contaminate a variety of processed meats. Microscopically, Listeria species appear as small, Gram-positive rods, which are sometimes arranged in short chains. In direct smears, they can be coccoid, so they can be mistaken for streptococci. Longer cells can resemble corynebacteria. Flagella are produced at room temperature but not at 37 degrees C. Haemolytic activity on blood agar has been used as a marker to distinguish Listeria monocytogenes among other Listeria species, but it is not an absolutely definitive criterion. Further biochemical characterization is necessary to distinguish between the different Listeria species. The objective of this study was to detect, isolate and identify Listeria monocytogenes from poultry meat. Within a period of six months from January to June 2017, a total of 15 samples were collected. Three samples were positive for the presence of Listeria monocytogenes. Biochemical and microbiological tests as well as PCR technique using specific primers were used to confirm L. Monocytogenes in the samples.", publisher = "Iop Publishing Ltd, Bristol", journal = "59th International Meat Industry Conference (MEATCON2017)", title = "Isolation and detection of Listeria monocytogenes in poultry meat by standard culture methods and PCR", volume = "85", pages = "UNSP 012069", doi = "10.1088/1755-1315/85/1/012069" }
Kureljušić, J., Rokvić, N. I., Jezdimirović, N., Kureljušić, B., Pisinov, B.,& Karabasil, N.. (2017). Isolation and detection of Listeria monocytogenes in poultry meat by standard culture methods and PCR. in 59th International Meat Industry Conference (MEATCON2017) Iop Publishing Ltd, Bristol., 85, UNSP 012069. https://doi.org/10.1088/1755-1315/85/1/012069
Kureljušić J, Rokvić NI, Jezdimirović N, Kureljušić B, Pisinov B, Karabasil N. Isolation and detection of Listeria monocytogenes in poultry meat by standard culture methods and PCR. in 59th International Meat Industry Conference (MEATCON2017). 2017;85:UNSP 012069. doi:10.1088/1755-1315/85/1/012069 .
Kureljušić, Jasna, Rokvić, Nikola I., Jezdimirović, Nemanja, Kureljušić, Branislav, Pisinov, Boris, Karabasil, Nedjeljko, "Isolation and detection of Listeria monocytogenes in poultry meat by standard culture methods and PCR" in 59th International Meat Industry Conference (MEATCON2017), 85 (2017):UNSP 012069, https://doi.org/10.1088/1755-1315/85/1/012069 . .