Influence of Environmental Conditions on Norovirus Presence in Mussels Harvested in Montenegro
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2017
Authors
Ilić, NevenaVelebit, Branko
Teodorović, Vlado
Đorđević, Vesna
Karabasil, Nedjeljko
Vasilev, Dragan
Đurić, Spomenka
Adzić, Bojan
Dimitrijević, Mirjana
Article (Published version)
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This study comprises the first systematic survey of the occurrence of Norovirus in Mediterranean mussels from harvesting areas in Montenegro coast of Adriatic Sea. Mussels may accumulate contaminants of public health concern, including pathogenic bacteria and viruses. Microbiological monitoring of harvesting areas is based on count of Escherichia coli in bivalve molluscs in the European Union. It is assumed that E. coli does not reflect contamination with enteric viruses. A structured field study was undertaken at six locations in Bay of Kotor, Montenegro, in order to investigate plausible influence of environmental factors on the variability of E. coli and norovirus (NoV). From July 2015 to July 2016, a total of 72 samples of mussels were collected in coastal harvesting areas of the Montenegro. The samples were screened for NoV of genogroups GI and GII using reverse transcription-qPCR (RT-qPCR). There were 43% NoV positive samples with higher presence of genogroup GII (74.2%). With re...gard to influence of environmental conditions on Norovirus presence, we have proved seasonal pattern of virus occurrence i.e., the largest number of positive samples was noticed during winter, while other physico-chemical factors were not of great significance. It was found that count of E. coli did not correlate with Norovirus prevalence. From the aspect of food safety, an upgrade of monitoring plans could lead to obtaining safer products.
Keywords:
Norovirus / Mussels / Environmental conditions / MontenegroSource:
Food and Environmental Virology, 2017, 9, 4, 406-414Publisher:
- Springer, New York
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
DOI: 10.1007/s12560-017-9298-0
ISSN: 1867-0334
PubMed: 28439785
WoS: 000414377300004
Scopus: 2-s2.0-85018841935
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Fakultet veterinarske medicineTY - JOUR AU - Ilić, Nevena AU - Velebit, Branko AU - Teodorović, Vlado AU - Đorđević, Vesna AU - Karabasil, Nedjeljko AU - Vasilev, Dragan AU - Đurić, Spomenka AU - Adzić, Bojan AU - Dimitrijević, Mirjana PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1540 AB - This study comprises the first systematic survey of the occurrence of Norovirus in Mediterranean mussels from harvesting areas in Montenegro coast of Adriatic Sea. Mussels may accumulate contaminants of public health concern, including pathogenic bacteria and viruses. Microbiological monitoring of harvesting areas is based on count of Escherichia coli in bivalve molluscs in the European Union. It is assumed that E. coli does not reflect contamination with enteric viruses. A structured field study was undertaken at six locations in Bay of Kotor, Montenegro, in order to investigate plausible influence of environmental factors on the variability of E. coli and norovirus (NoV). From July 2015 to July 2016, a total of 72 samples of mussels were collected in coastal harvesting areas of the Montenegro. The samples were screened for NoV of genogroups GI and GII using reverse transcription-qPCR (RT-qPCR). There were 43% NoV positive samples with higher presence of genogroup GII (74.2%). With regard to influence of environmental conditions on Norovirus presence, we have proved seasonal pattern of virus occurrence i.e., the largest number of positive samples was noticed during winter, while other physico-chemical factors were not of great significance. It was found that count of E. coli did not correlate with Norovirus prevalence. From the aspect of food safety, an upgrade of monitoring plans could lead to obtaining safer products. PB - Springer, New York T2 - Food and Environmental Virology T1 - Influence of Environmental Conditions on Norovirus Presence in Mussels Harvested in Montenegro VL - 9 IS - 4 SP - 406 EP - 414 DO - 10.1007/s12560-017-9298-0 ER -
@article{ author = "Ilić, Nevena and Velebit, Branko and Teodorović, Vlado and Đorđević, Vesna and Karabasil, Nedjeljko and Vasilev, Dragan and Đurić, Spomenka and Adzić, Bojan and Dimitrijević, Mirjana", year = "2017", abstract = "This study comprises the first systematic survey of the occurrence of Norovirus in Mediterranean mussels from harvesting areas in Montenegro coast of Adriatic Sea. Mussels may accumulate contaminants of public health concern, including pathogenic bacteria and viruses. Microbiological monitoring of harvesting areas is based on count of Escherichia coli in bivalve molluscs in the European Union. It is assumed that E. coli does not reflect contamination with enteric viruses. A structured field study was undertaken at six locations in Bay of Kotor, Montenegro, in order to investigate plausible influence of environmental factors on the variability of E. coli and norovirus (NoV). From July 2015 to July 2016, a total of 72 samples of mussels were collected in coastal harvesting areas of the Montenegro. The samples were screened for NoV of genogroups GI and GII using reverse transcription-qPCR (RT-qPCR). There were 43% NoV positive samples with higher presence of genogroup GII (74.2%). With regard to influence of environmental conditions on Norovirus presence, we have proved seasonal pattern of virus occurrence i.e., the largest number of positive samples was noticed during winter, while other physico-chemical factors were not of great significance. It was found that count of E. coli did not correlate with Norovirus prevalence. From the aspect of food safety, an upgrade of monitoring plans could lead to obtaining safer products.", publisher = "Springer, New York", journal = "Food and Environmental Virology", title = "Influence of Environmental Conditions on Norovirus Presence in Mussels Harvested in Montenegro", volume = "9", number = "4", pages = "406-414", doi = "10.1007/s12560-017-9298-0" }
Ilić, N., Velebit, B., Teodorović, V., Đorđević, V., Karabasil, N., Vasilev, D., Đurić, S., Adzić, B.,& Dimitrijević, M.. (2017). Influence of Environmental Conditions on Norovirus Presence in Mussels Harvested in Montenegro. in Food and Environmental Virology Springer, New York., 9(4), 406-414. https://doi.org/10.1007/s12560-017-9298-0
Ilić N, Velebit B, Teodorović V, Đorđević V, Karabasil N, Vasilev D, Đurić S, Adzić B, Dimitrijević M. Influence of Environmental Conditions on Norovirus Presence in Mussels Harvested in Montenegro. in Food and Environmental Virology. 2017;9(4):406-414. doi:10.1007/s12560-017-9298-0 .
Ilić, Nevena, Velebit, Branko, Teodorović, Vlado, Đorđević, Vesna, Karabasil, Nedjeljko, Vasilev, Dragan, Đurić, Spomenka, Adzić, Bojan, Dimitrijević, Mirjana, "Influence of Environmental Conditions on Norovirus Presence in Mussels Harvested in Montenegro" in Food and Environmental Virology, 9, no. 4 (2017):406-414, https://doi.org/10.1007/s12560-017-9298-0 . .