Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia
Samo za registrovane korisnike
2017
Autori
Bulajić, SnežanaČolović, Svetlana
Mišić, Dušan
Đorđević, Jasna
Savić Radovanović, Radoslava
Ašanin, Jelena
Ledina, Tijana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS (R) Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU... g(-1). Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia.
Ključne reči:
Antimicrobial resistance / enterotoxin production / Serbian raw milk cheeses / staphylococciIzvor:
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural, 2017, 52, 12, 864-870Izdavač:
- Taylor & Francis Inc, Philadelphia
Finansiranje / projekti:
- Unapređenje i razvoj higijenskih i tehnoloških postupaka u proizvodnji namirnica životinjskog porekla u cilju dobijanja kvalitetnih i bezbednih proizvoda konkurentnih na svetskom tržištu (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1080/03601234.2017.1361764
ISSN: 0360-1234
PubMed: 28949803
WoS: 000415764700004
Scopus: 2-s2.0-85029906100
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Bulajić, Snežana AU - Čolović, Svetlana AU - Mišić, Dušan AU - Đorđević, Jasna AU - Savić Radovanović, Radoslava AU - Ašanin, Jelena AU - Ledina, Tijana PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1542 AB - This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS (R) Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU g(-1). Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural T1 - Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia VL - 52 IS - 12 SP - 864 EP - 870 DO - 10.1080/03601234.2017.1361764 ER -
@article{ author = "Bulajić, Snežana and Čolović, Svetlana and Mišić, Dušan and Đorđević, Jasna and Savić Radovanović, Radoslava and Ašanin, Jelena and Ledina, Tijana", year = "2017", abstract = "This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS (R) Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU g(-1). Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural", title = "Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia", volume = "52", number = "12", pages = "864-870", doi = "10.1080/03601234.2017.1361764" }
Bulajić, S., Čolović, S., Mišić, D., Đorđević, J., Savić Radovanović, R., Ašanin, J.,& Ledina, T.. (2017). Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Taylor & Francis Inc, Philadelphia., 52(12), 864-870. https://doi.org/10.1080/03601234.2017.1361764
Bulajić S, Čolović S, Mišić D, Đorđević J, Savić Radovanović R, Ašanin J, Ledina T. Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural. 2017;52(12):864-870. doi:10.1080/03601234.2017.1361764 .
Bulajić, Snežana, Čolović, Svetlana, Mišić, Dušan, Đorđević, Jasna, Savić Radovanović, Radoslava, Ašanin, Jelena, Ledina, Tijana, "Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia" in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural, 52, no. 12 (2017):864-870, https://doi.org/10.1080/03601234.2017.1361764 . .