Investigation of aerobic microflora in bulls sperm

Abstract

Samples of native bulls sperm of Holstein - Friesian breed from Center for Artificial Insemination, were investigated. In bulls sperm numerous types of microorganisms are represented. Of conditionally pathogenic microorganisms Corynebacterium rénale and Staphylococcus auteur were isolated. By microbiological investigation it was established the important representation of saprophytic micro flora: Bacillus spp., Proteus mirabilis, coagulase of negative Staphylococcus, micrococcus, Aspergilus spp., etc. In technological process of seed treatment combinations of antibiotics of a large spectrum of influence are added. Taking into consideration that antimicrobial effect depends, first of all, on present microorganisms, it is clear why microbiological examination of semen as a part of healthy control of breeding bulls has a great importance for the success in cattle reproduction. .

Ispitivani su uzorci nativne i zamrznute sperme bikova Holštajn Frizijske rase Centra za veslačko osemenjavanje. U nativnoj spermi bikova zastupljene su brojne vrste mikroorganizama. Od uslovno patogenih mikroorganizama izolovani su Corynebacterium rénale i Staphylococcus aureus. Mikrobiološkom pretragom nativne sperme ustanovljena je značajna zastupljenost saprofitne mikroflore: Bacillus spp., Proteus mirabilis, koagulaza negativne stafilokoke, mikrokoke, Aspergilus spp., i dr. U tehnološkom procesu obrade semena dodaju se kombinacije antibiotika širokog spektra delovanja. Mikrobiološkom analizom uzoraka zamrznute sperme utvrđena je isključivo saprofitna mikroflora, dok je značajno veći broj uzoraka bio mikrobiološki negativan. Obzirom da antimikrobni efekat dodatih antibiotika putem razređivača, zavisi prevashodno od prisutnih mikroorganizama, jasno je zašto mikrobiološki pregled semena kao deo zdravstvene kontrole priplodnih bikova ima veliki značaj za uspeh u reprodukciji goveda. .