Indirect immunofluoroscence in evaluation of bulls sperm antigenic characteristics
Primena indirektne imunofluorescence u određivanju antigenskih karakteristika spermatozoida bika
Апстракт
Our investigations were conducted in order to evaluate variations in anti-sperm antibodies in rabbits sera. Rabbits were previously immunized with TRIS - egg yolk extender, Biociphos plus extender (IMV, France) and with content of insemination straws prepared with same extenders. We investigated sperm cells from ejaculates of 12 black and white spotted bulls by indirect immunofluorescence method. Both extenders and content of insemination straws were capable in raising antibody production. Titer of antibodies had the highest values when sperm cells from insemination straws were used for the test. .
U ovom radu su izneti rezultati ispitivanja varijacija u titru antitela protiv spermatozoida bika i razređivača za spermu bikova u serumu kunića imunizovanih TRIS žumanjčanim razređivačem, razređivačem Biociphos plus (IMV, France) i sadržajem pajeta za VO krava pripremljenim pomoću njih. Ispitivanja su vršena metodom indirektne imuno-fluorescence a ukupno je testirano 12 bikova crno-bele šarene rase. Postignuti rezultati ukazuju da oba razređivača dovode do nastanka antitela kod kunića a da je titar antitela na antigene spermatozoida značajno viši kada se za izvođenje testa koriste spermatozoidi iz pajeta za VO.
Кључне речи:
bull / sperm cells / antibody / extender / infertility / bik / spermatozoidi / antitela / razređivač / neplodnostИзвор:
Zbornik naučnih radova Instituta PKB Agroekonomik, 2000, 6, 1, 401-409Издавач:
- Institut PKB Agroekonomik, Padinska skela
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Lazarević, Miodrag AU - Rodić, Svetlana AU - Ostojić, Jelena AU - Jakovljević, Goran AU - Jovičin, Milovan PY - 2000 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/157 AB - Our investigations were conducted in order to evaluate variations in anti-sperm antibodies in rabbits sera. Rabbits were previously immunized with TRIS - egg yolk extender, Biociphos plus extender (IMV, France) and with content of insemination straws prepared with same extenders. We investigated sperm cells from ejaculates of 12 black and white spotted bulls by indirect immunofluorescence method. Both extenders and content of insemination straws were capable in raising antibody production. Titer of antibodies had the highest values when sperm cells from insemination straws were used for the test. . AB - U ovom radu su izneti rezultati ispitivanja varijacija u titru antitela protiv spermatozoida bika i razređivača za spermu bikova u serumu kunića imunizovanih TRIS žumanjčanim razređivačem, razređivačem Biociphos plus (IMV, France) i sadržajem pajeta za VO krava pripremljenim pomoću njih. Ispitivanja su vršena metodom indirektne imuno-fluorescence a ukupno je testirano 12 bikova crno-bele šarene rase. Postignuti rezultati ukazuju da oba razređivača dovode do nastanka antitela kod kunića a da je titar antitela na antigene spermatozoida značajno viši kada se za izvođenje testa koriste spermatozoidi iz pajeta za VO. PB - Institut PKB Agroekonomik, Padinska skela T2 - Zbornik naučnih radova Instituta PKB Agroekonomik T1 - Indirect immunofluoroscence in evaluation of bulls sperm antigenic characteristics T1 - Primena indirektne imunofluorescence u određivanju antigenskih karakteristika spermatozoida bika VL - 6 IS - 1 SP - 401 EP - 409 UR - https://hdl.handle.net/21.15107/rcub_veterinar_157 ER -
@article{ author = "Lazarević, Miodrag and Rodić, Svetlana and Ostojić, Jelena and Jakovljević, Goran and Jovičin, Milovan", year = "2000", abstract = "Our investigations were conducted in order to evaluate variations in anti-sperm antibodies in rabbits sera. Rabbits were previously immunized with TRIS - egg yolk extender, Biociphos plus extender (IMV, France) and with content of insemination straws prepared with same extenders. We investigated sperm cells from ejaculates of 12 black and white spotted bulls by indirect immunofluorescence method. Both extenders and content of insemination straws were capable in raising antibody production. Titer of antibodies had the highest values when sperm cells from insemination straws were used for the test. ., U ovom radu su izneti rezultati ispitivanja varijacija u titru antitela protiv spermatozoida bika i razređivača za spermu bikova u serumu kunića imunizovanih TRIS žumanjčanim razređivačem, razređivačem Biociphos plus (IMV, France) i sadržajem pajeta za VO krava pripremljenim pomoću njih. Ispitivanja su vršena metodom indirektne imuno-fluorescence a ukupno je testirano 12 bikova crno-bele šarene rase. Postignuti rezultati ukazuju da oba razređivača dovode do nastanka antitela kod kunića a da je titar antitela na antigene spermatozoida značajno viši kada se za izvođenje testa koriste spermatozoidi iz pajeta za VO.", publisher = "Institut PKB Agroekonomik, Padinska skela", journal = "Zbornik naučnih radova Instituta PKB Agroekonomik", title = "Indirect immunofluoroscence in evaluation of bulls sperm antigenic characteristics, Primena indirektne imunofluorescence u određivanju antigenskih karakteristika spermatozoida bika", volume = "6", number = "1", pages = "401-409", url = "https://hdl.handle.net/21.15107/rcub_veterinar_157" }
Lazarević, M., Rodić, S., Ostojić, J., Jakovljević, G.,& Jovičin, M.. (2000). Indirect immunofluoroscence in evaluation of bulls sperm antigenic characteristics. in Zbornik naučnih radova Instituta PKB Agroekonomik Institut PKB Agroekonomik, Padinska skela., 6(1), 401-409. https://hdl.handle.net/21.15107/rcub_veterinar_157
Lazarević M, Rodić S, Ostojić J, Jakovljević G, Jovičin M. Indirect immunofluoroscence in evaluation of bulls sperm antigenic characteristics. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2000;6(1):401-409. https://hdl.handle.net/21.15107/rcub_veterinar_157 .
Lazarević, Miodrag, Rodić, Svetlana, Ostojić, Jelena, Jakovljević, Goran, Jovičin, Milovan, "Indirect immunofluoroscence in evaluation of bulls sperm antigenic characteristics" in Zbornik naučnih radova Instituta PKB Agroekonomik, 6, no. 1 (2000):401-409, https://hdl.handle.net/21.15107/rcub_veterinar_157 .