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dc.creatorGavrilović, A.
dc.creatorGavrilović, P.
dc.creatorRadojičić, Sonja
dc.creatorKrnjaić, Dejan
dc.date.accessioned2020-06-03T14:21:26Z
dc.date.available2020-06-03T14:21:26Z
dc.date.issued2018
dc.identifier.issn1792-2720
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1570
dc.description.abstractBluetongue (BT) is a viral non-contagious disease of ruminants which is transmitted by insects of the genus Culicoides. In recent years, BT has been a serious threat to livestock and to the economies of European countries. In Serbia the disease appeared for the first time in 2001, and after a 12 year period of freedom, it broke out again in 2014. Considering the actuality of this infectious disease, especially the need for prompt and rapid diagnostics, the aim of this paper was to determine the possibility of detecting the serological response in sheep and cattle with manifested clinical signs of the disease using two different methods: double recognition enzyme-linked immunosorbent assay (sELISA) and competitive enzyme-linked immunosorbent assay (cELISA). A total of 105 blood serum samples of cattle and sheep, which had exhibited clinical signs of BT during 2014, were taken for examination from a serum bank. Out of 74 blood serum samples of sheep and 31 blood serum samples of cattle, 52 samples of sheep and 18 samples of cattle tested positive using sELISA, while 50 samples of sheep and 18 samples of cattle gave positive reactions with cELISA. The results confirm the high sensitivity of sELISA which detected 4% more seropositive sheep in comparison with cELISA. Using Cohens kappa statistical analysis, almost perfect agreement was determined between the results (k>0,81) obtained by cELISA and sELISA.en
dc.publisherHellenic Veterinary Medical Soc, Athens
dc.rightsopenAccess
dc.sourceJournal of The Hellenic Veterinary Medical Society
dc.subjectbluetongueen
dc.subjectcELISAen
dc.subjectsELISAen
dc.subjectruminantsen
dc.subjectSerbiaen
dc.titleComparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assaysen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГавриловић, A.; Гавриловић, П.; Крњаић, Дејан; Радојичић, Соња;
dc.citation.volume69
dc.citation.issue3
dc.citation.spage1088
dc.citation.epage1093
dc.citation.other69(3): 1088-1093
dc.citation.rankM23
dc.identifier.wos000452920000008
dc.identifier.doi10.12681/jhvms.18880
dc.identifier.scopus2-s2.0-85057496527
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/533/1569.pdf
dc.identifier.rcubconv_2444
dc.type.versionpublishedVersion


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