Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study
Samo za registrovane korisnike
2018
Autori
Cetenović, BojanaColović, Bozana
Vasilijić, Sasa
Prokić, Bogomir Bolka
Pasalić, Snežana
Jokanović, Vukoman
Tepavčević, Zvezdana
Marković, Dejan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials suspensions were conducted by pH-meter. The cytotoxicity of materials solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, wh...ile in case of MTA(+) they were lower and continuously declined (p<0.05). Investigated materials didnt exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p<0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p<0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .
Izvor:
Journal of Materials Science-Materials in Medicine, 2018, 29, 12, 190-Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Hemijsko i strukturno dizajniranje nanomaterijala za primenu u medicini i inženjerstvu tkiva (RS-MESTD-Basic Research (BR or ON)-172026)
DOI: 10.1007/s10856-018-6200-z
ISSN: 0957-4530
PubMed: 30536136
WoS: 000452711900004
Scopus: 2-s2.0-85058111668
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Cetenović, Bojana AU - Colović, Bozana AU - Vasilijić, Sasa AU - Prokić, Bogomir Bolka AU - Pasalić, Snežana AU - Jokanović, Vukoman AU - Tepavčević, Zvezdana AU - Marković, Dejan PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1571 AB - The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials suspensions were conducted by pH-meter. The cytotoxicity of materials solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p<0.05). Investigated materials didnt exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p<0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p<0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] . PB - Springer, Dordrecht T2 - Journal of Materials Science-Materials in Medicine T1 - Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study VL - 29 IS - 12 SP - 190 DO - 10.1007/s10856-018-6200-z ER -
@article{ author = "Cetenović, Bojana and Colović, Bozana and Vasilijić, Sasa and Prokić, Bogomir Bolka and Pasalić, Snežana and Jokanović, Vukoman and Tepavčević, Zvezdana and Marković, Dejan", year = "2018", abstract = "The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials suspensions were conducted by pH-meter. The cytotoxicity of materials solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p<0.05). Investigated materials didnt exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p<0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p<0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .", publisher = "Springer, Dordrecht", journal = "Journal of Materials Science-Materials in Medicine", title = "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study", volume = "29", number = "12", pages = "190", doi = "10.1007/s10856-018-6200-z" }
Cetenović, B., Colović, B., Vasilijić, S., Prokić, B. B., Pasalić, S., Jokanović, V., Tepavčević, Z.,& Marković, D.. (2018). Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine Springer, Dordrecht., 29(12), 190. https://doi.org/10.1007/s10856-018-6200-z
Cetenović B, Colović B, Vasilijić S, Prokić BB, Pasalić S, Jokanović V, Tepavčević Z, Marković D. Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine. 2018;29(12):190. doi:10.1007/s10856-018-6200-z .
Cetenović, Bojana, Colović, Bozana, Vasilijić, Sasa, Prokić, Bogomir Bolka, Pasalić, Snežana, Jokanović, Vukoman, Tepavčević, Zvezdana, Marković, Dejan, "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study" in Journal of Materials Science-Materials in Medicine, 29, no. 12 (2018):190, https://doi.org/10.1007/s10856-018-6200-z . .