Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia
2018
Аутори
Gavrilović, MilanTešević, Vele
Đorđević, Iris
Rajcević, Nemanja
Bakhia, Arsena
Garcia Jacas, Nuria
Susanna, Alfonso
Marin, Petar D.
Janaćković, Peđa
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH 91,1-diphenyl-2-picrylhydrazyl) radical and the 2,2-azino-bis-3-ethylbenzthiazoline-6-sulph...onic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine.
Кључне речи:
Amphoricarpos elegans / glandular trichomes / 3-deoxyamphoricarpolide / antioxidative activityИзвор:
Archives of Biological Sciences, 2018, 70, 4, 613-620Издавач:
- Srpsko biološko društvo, Beograd
Финансирање / пројекти:
- Микроморфолошка, фитохемијска и молекуларна истраживања биљака - систематски, еколошки и применљиви аспекти (RS-173029)
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-172053)
DOI: 10.2298/ABS180309022G
ISSN: 0354-4664
WoS: 000452187000002
Scopus: 2-s2.0-85058801815
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Gavrilović, Milan AU - Tešević, Vele AU - Đorđević, Iris AU - Rajcević, Nemanja AU - Bakhia, Arsena AU - Garcia Jacas, Nuria AU - Susanna, Alfonso AU - Marin, Petar D. AU - Janaćković, Peđa PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1575 AB - We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH 91,1-diphenyl-2-picrylhydrazyl) radical and the 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine. PB - Srpsko biološko društvo, Beograd T2 - Archives of Biological Sciences T1 - Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia VL - 70 IS - 4 SP - 613 EP - 620 DO - 10.2298/ABS180309022G ER -
@article{ author = "Gavrilović, Milan and Tešević, Vele and Đorđević, Iris and Rajcević, Nemanja and Bakhia, Arsena and Garcia Jacas, Nuria and Susanna, Alfonso and Marin, Petar D. and Janaćković, Peđa", year = "2018", abstract = "We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH 91,1-diphenyl-2-picrylhydrazyl) radical and the 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine.", publisher = "Srpsko biološko društvo, Beograd", journal = "Archives of Biological Sciences", title = "Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia", volume = "70", number = "4", pages = "613-620", doi = "10.2298/ABS180309022G" }
Gavrilović, M., Tešević, V., Đorđević, I., Rajcević, N., Bakhia, A., Garcia Jacas, N., Susanna, A., Marin, P. D.,& Janaćković, P.. (2018). Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia. in Archives of Biological Sciences Srpsko biološko društvo, Beograd., 70(4), 613-620. https://doi.org/10.2298/ABS180309022G
Gavrilović M, Tešević V, Đorđević I, Rajcević N, Bakhia A, Garcia Jacas N, Susanna A, Marin PD, Janaćković P. Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia. in Archives of Biological Sciences. 2018;70(4):613-620. doi:10.2298/ABS180309022G .
Gavrilović, Milan, Tešević, Vele, Đorđević, Iris, Rajcević, Nemanja, Bakhia, Arsena, Garcia Jacas, Nuria, Susanna, Alfonso, Marin, Petar D., Janaćković, Peđa, "Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia" in Archives of Biological Sciences, 70, no. 4 (2018):613-620, https://doi.org/10.2298/ABS180309022G . .