Indirect immunofluoroscence in evaluation of bulls sperm antigenic characteristics

Abstract

Our investigations were conducted in order to evaluate variations in anti-sperm antibodies in rabbits sera. Rabbits were previously immunized with TRIS - egg yolk extender, Biociphos plus extender (IMV, France) and with content of insemination straws prepared with same extenders. We investigated sperm cells from ejaculates of 12 black and white spotted bulls by indirect immunofluorescence method. Both extenders and content of insemination straws were capable in raising antibody production. Titer of antibodies had the highest values when sperm cells from insemination straws were used for the test. .

U ovom radu su izneti rezultati ispitivanja varijacija u titru antitela protiv spermatozoida bika i razređivača za spermu bikova u serumu kunića imunizovanih TRIS žumanjčanim razređivačem, razređivačem Biociphos plus (IMV, France) i sadržajem pajeta za VO krava pripremljenim pomoću njih. Ispitivanja su vršena metodom indirektne imuno-fluorescence a ukupno je testirano 12 bikova crno-bele šarene rase. Postignuti rezultati ukazuju da oba razređivača dovode do nastanka antitela kod kunića a da je titar antitela na antigene spermatozoida značajno viši kada se za izvođenje testa koriste spermatozoidi iz pajeta za VO.