Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
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2018
Authors
Stojanović, M.Todorović, D.
Šćepanović, Ljiljana
Mitrović, D.
Borozan, Sunčica
Dragutinović, Vesna
Labudović-Borović, Milica
Krstić, D.
Colović, M.
Đurić, D.
Article (Published version)
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The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that C...ys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
Keywords:
Methionine / Antioxidants / Morphometry / Biochemical analysis / Liver / RatSource:
Molecular and Cellular Biochemistry, 2018, 448, 1-2, 43-50Publisher:
- Springer, Dordrecht
Funding / projects:
- The effects of homocysteine and homocysteine-related compounds on cardiovascular system: role of gaseous transmitters No, H2S and CO (RS-MESTD-Basic Research (BR or ON)-175043)
DOI: 10.1007/s11010-018-3311-2
ISSN: 0300-8177
PubMed: 29423685
WoS: 000446385800005
Scopus: 2-s2.0-85041517196
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Fakultet veterinarske medicineTY - JOUR AU - Stojanović, M. AU - Todorović, D. AU - Šćepanović, Ljiljana AU - Mitrović, D. AU - Borozan, Sunčica AU - Dragutinović, Vesna AU - Labudović-Borović, Milica AU - Krstić, D. AU - Colović, M. AU - Đurić, D. PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1585 AB - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure. PB - Springer, Dordrecht T2 - Molecular and Cellular Biochemistry T1 - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue VL - 448 IS - 1-2 SP - 43 EP - 50 DO - 10.1007/s11010-018-3311-2 ER -
@article{ author = "Stojanović, M. and Todorović, D. and Šćepanović, Ljiljana and Mitrović, D. and Borozan, Sunčica and Dragutinović, Vesna and Labudović-Borović, Milica and Krstić, D. and Colović, M. and Đurić, D.", year = "2018", abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.", publisher = "Springer, Dordrecht", journal = "Molecular and Cellular Biochemistry", title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue", volume = "448", number = "1-2", pages = "43-50", doi = "10.1007/s11010-018-3311-2" }
Stojanović, M., Todorović, D., Šćepanović, L., Mitrović, D., Borozan, S., Dragutinović, V., Labudović-Borović, M., Krstić, D., Colović, M.,& Đurić, D.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry Springer, Dordrecht., 448(1-2), 43-50. https://doi.org/10.1007/s11010-018-3311-2
Stojanović M, Todorović D, Šćepanović L, Mitrović D, Borozan S, Dragutinović V, Labudović-Borović M, Krstić D, Colović M, Đurić D. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50. doi:10.1007/s11010-018-3311-2 .
Stojanović, M., Todorović, D., Šćepanović, Ljiljana, Mitrović, D., Borozan, Sunčica, Dragutinović, Vesna, Labudović-Borović, Milica, Krstić, D., Colović, M., Đurić, D., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50, https://doi.org/10.1007/s11010-018-3311-2 . .