The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis
2018
Аутори
Polaček, VladimirVidanović, Dejan
Božić, B.
Becskei, Zsolt
Vučićević, Ivana
Prodanov-Radulović, Jasna
Aleksić-Kovačević, Sanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. h...ominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.
Кључне речи:
Granuloma / Mycobacterium avium subsp. hominissuis / Myofibroblast / TGF-β1 / TGF-β1RIИзвор:
Macedonian Veterinary Review, 2018, 41, 1, 47-53Издавач:
- Faculty of Veterinary Medicine - Skopje
Финансирање / пројекти:
- Истраживање фармаколошких карактеристика антимикробних агенаса, увођење нових технолошких решења и алтернативних метода профилаксе с циљем да се побољша контрола инфективних обољења домаћих животиња (RS-MESTD-Technological Development (TD or TR)-31071)
- Праћење здравственог стања дивљачи и увођење нових биотехнолошких поступака у детекцији заразних и зоонозних агенаса - анализа ризика за здравље људи, домаћих и дивљих животиња и контаминацију животне средине (RS-MESTD-Technological Development (TD or TR)-31084)
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Polaček, Vladimir AU - Vidanović, Dejan AU - Božić, B. AU - Becskei, Zsolt AU - Vučićević, Ivana AU - Prodanov-Radulović, Jasna AU - Aleksić-Kovačević, Sanja PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1598 AB - The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH. PB - Faculty of Veterinary Medicine - Skopje T2 - Macedonian Veterinary Review T1 - The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis VL - 41 IS - 1 SP - 47 EP - 53 DO - 10.1515/macvetrev-2017-0030 ER -
@article{ author = "Polaček, Vladimir and Vidanović, Dejan and Božić, B. and Becskei, Zsolt and Vučićević, Ivana and Prodanov-Radulović, Jasna and Aleksić-Kovačević, Sanja", year = "2018", abstract = "The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.", publisher = "Faculty of Veterinary Medicine - Skopje", journal = "Macedonian Veterinary Review", title = "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis", volume = "41", number = "1", pages = "47-53", doi = "10.1515/macvetrev-2017-0030" }
Polaček, V., Vidanović, D., Božić, B., Becskei, Z., Vučićević, I., Prodanov-Radulović, J.,& Aleksić-Kovačević, S.. (2018). The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review Faculty of Veterinary Medicine - Skopje., 41(1), 47-53. https://doi.org/10.1515/macvetrev-2017-0030
Polaček V, Vidanović D, Božić B, Becskei Z, Vučićević I, Prodanov-Radulović J, Aleksić-Kovačević S. The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review. 2018;41(1):47-53. doi:10.1515/macvetrev-2017-0030 .
Polaček, Vladimir, Vidanović, Dejan, Božić, B., Becskei, Zsolt, Vučićević, Ivana, Prodanov-Radulović, Jasna, Aleksić-Kovačević, Sanja, "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis" in Macedonian Veterinary Review, 41, no. 1 (2018):47-53, https://doi.org/10.1515/macvetrev-2017-0030 . .