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dc.creatorTopalović, Dijana
dc.creatorDekanski, Dragana
dc.creatorSpremo-Potparević, Biljana
dc.creatorĐelić, Ninoslav
dc.creatorBajić, Vladan
dc.creatorŽivković, Lada
dc.date.accessioned2020-06-03T14:24:54Z
dc.date.available2020-06-03T14:24:54Z
dc.date.issued2018
dc.identifier.issn0004-1254
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1619
dc.description.abstractHarmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.en
dc.publisherInst Medical Research & Occupational Health, Zagreb
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//
dc.relationhCOMET COSTEuropean Cooperation in Science and Technology (COST)
dc.rightsopenAccess
dc.sourceArhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
dc.subjectDNA repairen
dc.subjecthydrogen peroxideen
dc.subjectgenotoxicityen
dc.subjectquercetinen
dc.subjectROSen
dc.titleAssessment of adrenaline-induced DNA damage in whole blood cells with the comet assayen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractТопаловић, Дијана; Спремо-Потпаревић, Биљана; Живковић, Лада; Бајић, Владан; Декански, Драгана; Ђелић, Нинослав;
dc.citation.volume69
dc.citation.issue4
dc.citation.spage304
dc.citation.epage308
dc.citation.other69(4): 304-308
dc.citation.rankM23
dc.identifier.wos000455487400003
dc.identifier.doi10.2478/aiht-2018-69-3154
dc.identifier.pmid30864376
dc.identifier.scopus2-s2.0-85062834853
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/580/1618.pdf
dc.identifier.rcubconv_2452
dc.type.versionpublishedVersion


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