Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia
Само за регистроване кориснике
2018
Аутори
Vasić, AnaNieder, Marion
Zdravković, Nemanja
Bojkovski, Jovan
Bugarski, Dejan
Pavlović, Ivan
Silaghi, Cornelia
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of A. phagocytophilum was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus (n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. marginatus ...(n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific antibodies by IFAT. However, the 135 blood samples tested for A. phagocytophilum DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign Theileria spp. with 100% identity with GenBank entries from Italy (Theileria sergenti), China (Theileria spp.), and Korea (Theileria buffeli isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens Theileria spp. and A. phagocytophilum.
Кључне речи:
Dairy cows / Tick-borne pathogens / Ixodes ricinus / Dermacentor marginatus / Haemaphysalis punctata / SerbiaИзвор:
Parasitology Research, 2018, 117, 6, 1813-1818Издавач:
- Springer, New York
Финансирање / пројекти:
- German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD)
DOI: 10.1007/s00436-018-5867-x
ISSN: 0932-0113
PubMed: 29679202
WoS: 000432128400016
Scopus: 2-s2.0-85045744388
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Vasić, Ana AU - Nieder, Marion AU - Zdravković, Nemanja AU - Bojkovski, Jovan AU - Bugarski, Dejan AU - Pavlović, Ivan AU - Silaghi, Cornelia PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1625 AB - Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of A. phagocytophilum was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus (n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. marginatus (n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific antibodies by IFAT. However, the 135 blood samples tested for A. phagocytophilum DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign Theileria spp. with 100% identity with GenBank entries from Italy (Theileria sergenti), China (Theileria spp.), and Korea (Theileria buffeli isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens Theileria spp. and A. phagocytophilum. PB - Springer, New York T2 - Parasitology Research T1 - Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia VL - 117 IS - 6 SP - 1813 EP - 1818 DO - 10.1007/s00436-018-5867-x ER -
@article{ author = "Vasić, Ana and Nieder, Marion and Zdravković, Nemanja and Bojkovski, Jovan and Bugarski, Dejan and Pavlović, Ivan and Silaghi, Cornelia", year = "2018", abstract = "Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of A. phagocytophilum was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus (n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. marginatus (n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific antibodies by IFAT. However, the 135 blood samples tested for A. phagocytophilum DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign Theileria spp. with 100% identity with GenBank entries from Italy (Theileria sergenti), China (Theileria spp.), and Korea (Theileria buffeli isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens Theileria spp. and A. phagocytophilum.", publisher = "Springer, New York", journal = "Parasitology Research", title = "Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia", volume = "117", number = "6", pages = "1813-1818", doi = "10.1007/s00436-018-5867-x" }
Vasić, A., Nieder, M., Zdravković, N., Bojkovski, J., Bugarski, D., Pavlović, I.,& Silaghi, C.. (2018). Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia. in Parasitology Research Springer, New York., 117(6), 1813-1818. https://doi.org/10.1007/s00436-018-5867-x
Vasić A, Nieder M, Zdravković N, Bojkovski J, Bugarski D, Pavlović I, Silaghi C. Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia. in Parasitology Research. 2018;117(6):1813-1818. doi:10.1007/s00436-018-5867-x .
Vasić, Ana, Nieder, Marion, Zdravković, Nemanja, Bojkovski, Jovan, Bugarski, Dejan, Pavlović, Ivan, Silaghi, Cornelia, "Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia" in Parasitology Research, 117, no. 6 (2018):1813-1818, https://doi.org/10.1007/s00436-018-5867-x . .