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dc.creatorRadalj, Andrea
dc.creatorNišavić, Jakov
dc.creatorKrnjaić, Dejan
dc.creatorValčić, Miroslav
dc.creatorJovanović, Tanja
dc.creatorVeljović, Ljubiša
dc.creatorMilić, Nenad
dc.date.accessioned2020-06-03T14:25:25Z
dc.date.available2020-06-03T14:25:25Z
dc.date.issued2018
dc.identifier.issn0001-7213
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1626
dc.description.abstractThe presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.en
dc.publisherVeterinarni A Farmaceuticka Univerzita Brno, Brno
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31008/RS//
dc.rightsopenAccess
dc.sourceActa veterinaria - Brno
dc.subjectEHV-1en
dc.subjectEHV-2en
dc.subjectEHV-5en
dc.subjectvirus isolationen
dc.subjectmolecular characterizationen
dc.titleDetection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbiaen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКрњаић, Дејан; Милић, Ненад; Вељовић, Љубиша; Радаљ, Aндреа; Нишавић, Јаков; Валчић, Мирослав; Јовановић, Тања;
dc.citation.volume87
dc.citation.issue1
dc.citation.spage27
dc.citation.other87(1): 27-
dc.citation.rankM23
dc.identifier.wos000431956300004
dc.identifier.doi10.2754/avb201887010027
dc.identifier.scopus2-s2.0-85045414513
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/586/1625.pdf
dc.identifier.rcubconv_2375
dc.type.versionpublishedVersion


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