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Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia
dc.creator | Radalj, Andrea | |
dc.creator | Nišavić, Jakov | |
dc.creator | Krnjaić, Dejan | |
dc.creator | Valčić, Miroslav | |
dc.creator | Jovanović, Tanja | |
dc.creator | Veljović, Ljubiša | |
dc.creator | Milić, Nenad | |
dc.date.accessioned | 2020-06-03T14:25:25Z | |
dc.date.available | 2020-06-03T14:25:25Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0001-7213 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/1626 | |
dc.description.abstract | The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains. | en |
dc.publisher | Veterinarni A Farmaceuticka Univerzita Brno, Brno | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31008/RS// | |
dc.rights | openAccess | |
dc.source | Acta Veterinaria - Brno | |
dc.subject | EHV-1 | en |
dc.subject | EHV-2 | en |
dc.subject | EHV-5 | en |
dc.subject | virus isolation | en |
dc.subject | molecular characterization | en |
dc.title | Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Крњаић, Дејан; Милић, Ненад; Вељовић, Љубиша; Радаљ, Aндреа; Нишавић, Јаков; Валчић, Мирослав; Јовановић, Тања; | |
dc.citation.volume | 87 | |
dc.citation.issue | 1 | |
dc.citation.spage | 27 | |
dc.citation.other | 87(1): 27- | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000431956300004 | |
dc.identifier.doi | 10.2754/avb201887010027 | |
dc.identifier.scopus | 2-s2.0-85045414513 | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/586/1625.pdf | |
dc.type.version | publishedVersion |