Приказ основних података о документу
The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
dc.creator | Pantelić, M. | |
dc.creator | Jovanović, Ljubomir | |
dc.creator | Prodanović, Radiša | |
dc.creator | Vujanac, Ivan | |
dc.creator | Durić, M. | |
dc.creator | Culafić, T. | |
dc.creator | Vranješ-Đurić, Sanja | |
dc.creator | Korićanac, Goran | |
dc.creator | Kirovski, Danijela | |
dc.date.accessioned | 2020-06-03T14:25:39Z | |
dc.date.available | 2020-06-03T14:25:39Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0931-2439 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/1629 | |
dc.description.abstract | Thirty days before expected time of parturition, 20 Holstein cows were divided into -Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days -30, -10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days -10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days -28, -7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of beta-subunit of insulin receptor (IR beta) was significantly higher (p < 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser(307)) was significantly lower (p < 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser(307) was significantly lower in muscle tissue at days 7 and 28 (p < 0.05 and p < 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser(473)) was significantly higher (p < 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41009/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Animal Physiology and Animal Nutrition | |
dc.subject | bovine | en |
dc.subject | chromium | en |
dc.subject | insulin signalling pathway | en |
dc.title | The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Врањеш-Ђурић, Сања; Пантелић, М.; Јовановић, Љубомир; Дурић, М.; Корићанац, Горан; Вујанац, Иван; Продановић, Радиша; Цулафић, Т.; Кировски, Данијела; | |
dc.citation.volume | 102 | |
dc.citation.issue | 1 | |
dc.citation.spage | 41 | |
dc.citation.epage | 55 | |
dc.citation.other | 102(1): 41-55 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000430374000064 | |
dc.identifier.doi | 10.1111/jpn.12655 | |
dc.identifier.pmid | 28133865 | |
dc.identifier.scopus | 2-s2.0-85010988840 | |
dc.type.version | publishedVersion |