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dc.creatorLedina, Tijana
dc.creatorMohar-Lorbeg, Petra
dc.creatorGolob, Majda
dc.creatorĐorđević, Jasna
dc.creatorBogović-Matijasić, Bojana
dc.creatorBulajić, Snežana
dc.date.accessioned2020-06-03T14:26:06Z
dc.date.available2020-06-03T14:26:06Z
dc.date.issued2018
dc.identifier.issn0022-1155
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1635
dc.description.abstractThe aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed.en
dc.publisherSpringer India, New Delhi
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46009/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsopenAccess
dc.sourceJournal of Food Science and Technology-Mysore
dc.subjectAntibiotic resistanceen
dc.subjectTetracyclineen
dc.subjectLactobacillien
dc.subjectRaw milk cheeseen
dc.subjectMALDI TOF mass spectrometryen
dc.subjectRep-PCRen
dc.titleTetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheesesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБоговић-Матијасић, Бојана; Булајић, Снежана; Ђорђевић, Јасна; Ледина, Тијана; Голоб, Мајда; Мохар-Лорбег, Петра;
dc.citation.volume55
dc.citation.issue4
dc.citation.spage1426
dc.citation.epage1434
dc.citation.other55(4): 1426-1434
dc.citation.rankM22
dc.identifier.wos000428849100022
dc.identifier.doi10.1007/s13197-018-3057-6
dc.identifier.pmid29606757
dc.identifier.scopus2-s2.0-85044647859
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/591/1634.pdf
dc.identifier.rcubconv_2363
dc.type.versionpublishedVersion


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