Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs
Samo za registrovane korisnike
2018
Autori
Drašković, VladimirBošnjak-Neumuller, Jasna
Vasiljević, Marko
Petrujkić, Branko
Aleksić, Nevenka
Kukolj, Vladimir
Stanimirović, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Lawsonia intracellularis is known to cause proliferative enteropathy (PE), one of the economically most important swine diseases with global distribution. Not unlike other enteric diseases, PE is a frequent indication for antibiotic therapy. However, their unjustified use leads to an emerging problem antimicrobial resistance. Thus, the aim of this research was to assess if a phytogenic additive may replace antibiotics in the control of PE in 144 weaned piglets (72 treated and 72 controls) naturally infected with L. intracellularis. The quantity of L intracellularis faecal shedding was monitored by real-time polymerase chain reaction (PCR) assay in faecal samples on day 0, 14 and 28, whilst the level of the ileum damage was determined by immunohistochemistry (IHC) assay performed on gut sections. Real-time PCR assay revealed that cycle-threshold (Ct) values in the treatment group increased significantly over time and were higher than in the control. These results indicate that the use o...f the phytogenic additive decreases the faecal excretion of L. intracellularis both throughout the experiment and in comparison to the control. The expression of the L. intracellularis antigen in IHC assay was lower in treated animals, implying that the additive leads to the decrease in the pathogen quantity in the ileum. Significantly higher feed conversion ratio was recorded in the treatment group. The results indicate that the phytogenic additive may be beneficial in the control of PE, but additional research is necessary to assess its use in various pig categories and define the optimum concentrations.
Ključne reči:
Lawsonia intracellularis / Proliferative enteropathy / Phytogenic additive / PigIzvor:
Preventive Veterinary Medicine, 2018, 151, 46-51Izdavač:
- Elsevier Science Bv, Amsterdam
Finansiranje / projekti:
- Molekularno-genetička i ekofiziološka istraživanja u zaštiti autohtonih animalnih genetičkih resursa, očuvanju dobrobiti, zdravlja i reprodukcije gajenih životinja i proizvodnji bezbedne hrane (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1016/j.prevetmed.2018.01.002
ISSN: 0167-5877
PubMed: 29496105
WoS: 000428098300007
Scopus: 2-s2.0-85042927308
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Drašković, Vladimir AU - Bošnjak-Neumuller, Jasna AU - Vasiljević, Marko AU - Petrujkić, Branko AU - Aleksić, Nevenka AU - Kukolj, Vladimir AU - Stanimirović, Zoran PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1636 AB - Lawsonia intracellularis is known to cause proliferative enteropathy (PE), one of the economically most important swine diseases with global distribution. Not unlike other enteric diseases, PE is a frequent indication for antibiotic therapy. However, their unjustified use leads to an emerging problem antimicrobial resistance. Thus, the aim of this research was to assess if a phytogenic additive may replace antibiotics in the control of PE in 144 weaned piglets (72 treated and 72 controls) naturally infected with L. intracellularis. The quantity of L intracellularis faecal shedding was monitored by real-time polymerase chain reaction (PCR) assay in faecal samples on day 0, 14 and 28, whilst the level of the ileum damage was determined by immunohistochemistry (IHC) assay performed on gut sections. Real-time PCR assay revealed that cycle-threshold (Ct) values in the treatment group increased significantly over time and were higher than in the control. These results indicate that the use of the phytogenic additive decreases the faecal excretion of L. intracellularis both throughout the experiment and in comparison to the control. The expression of the L. intracellularis antigen in IHC assay was lower in treated animals, implying that the additive leads to the decrease in the pathogen quantity in the ileum. Significantly higher feed conversion ratio was recorded in the treatment group. The results indicate that the phytogenic additive may be beneficial in the control of PE, but additional research is necessary to assess its use in various pig categories and define the optimum concentrations. PB - Elsevier Science Bv, Amsterdam T2 - Preventive Veterinary Medicine T1 - Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs VL - 151 SP - 46 EP - 51 DO - 10.1016/j.prevetmed.2018.01.002 ER -
@article{ author = "Drašković, Vladimir and Bošnjak-Neumuller, Jasna and Vasiljević, Marko and Petrujkić, Branko and Aleksić, Nevenka and Kukolj, Vladimir and Stanimirović, Zoran", year = "2018", abstract = "Lawsonia intracellularis is known to cause proliferative enteropathy (PE), one of the economically most important swine diseases with global distribution. Not unlike other enteric diseases, PE is a frequent indication for antibiotic therapy. However, their unjustified use leads to an emerging problem antimicrobial resistance. Thus, the aim of this research was to assess if a phytogenic additive may replace antibiotics in the control of PE in 144 weaned piglets (72 treated and 72 controls) naturally infected with L. intracellularis. The quantity of L intracellularis faecal shedding was monitored by real-time polymerase chain reaction (PCR) assay in faecal samples on day 0, 14 and 28, whilst the level of the ileum damage was determined by immunohistochemistry (IHC) assay performed on gut sections. Real-time PCR assay revealed that cycle-threshold (Ct) values in the treatment group increased significantly over time and were higher than in the control. These results indicate that the use of the phytogenic additive decreases the faecal excretion of L. intracellularis both throughout the experiment and in comparison to the control. The expression of the L. intracellularis antigen in IHC assay was lower in treated animals, implying that the additive leads to the decrease in the pathogen quantity in the ileum. Significantly higher feed conversion ratio was recorded in the treatment group. The results indicate that the phytogenic additive may be beneficial in the control of PE, but additional research is necessary to assess its use in various pig categories and define the optimum concentrations.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Preventive Veterinary Medicine", title = "Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs", volume = "151", pages = "46-51", doi = "10.1016/j.prevetmed.2018.01.002" }
Drašković, V., Bošnjak-Neumuller, J., Vasiljević, M., Petrujkić, B., Aleksić, N., Kukolj, V.,& Stanimirović, Z.. (2018). Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs. in Preventive Veterinary Medicine Elsevier Science Bv, Amsterdam., 151, 46-51. https://doi.org/10.1016/j.prevetmed.2018.01.002
Drašković V, Bošnjak-Neumuller J, Vasiljević M, Petrujkić B, Aleksić N, Kukolj V, Stanimirović Z. Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs. in Preventive Veterinary Medicine. 2018;151:46-51. doi:10.1016/j.prevetmed.2018.01.002 .
Drašković, Vladimir, Bošnjak-Neumuller, Jasna, Vasiljević, Marko, Petrujkić, Branko, Aleksić, Nevenka, Kukolj, Vladimir, Stanimirović, Zoran, "Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs" in Preventive Veterinary Medicine, 151 (2018):46-51, https://doi.org/10.1016/j.prevetmed.2018.01.002 . .