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dc.creatorVejnović, Branislav
dc.creatorStevanović, Jevrosima
dc.creatorSchwarz, Ryan S.
dc.creatorAleksić, Nevenka
dc.creatorMirilović, Milorad
dc.creatorJovanović, Nemanja M.
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T14:26:27Z
dc.date.available2020-06-03T14:26:27Z
dc.date.issued2018
dc.identifier.issn0022-2011
dc.identifier.urihttps://vet-erinar.vet.bg.ac.rs/handle/123456789/1640
dc.description.abstractA recently described trypanosomatid species Lotmaria passim and the microsporidium Nosema ceranae infect the honey bee (Apis mellifera), but the interspecific dynamic of these two common gut parasites is unknown. In this study, a real-time qPCR assay was developed to enable the specific detection and quantification of L. passim. The annual dynamics of N. ceranae and L. passim infections were evaluated in ten A. mellifera colonies naturally infected with both parasites at one apiary in Serbia from March 2016 to March 2017. Ten samples (60 bees abdomens) were taken from each colony on 8 sampling occasions. L. passim infection level was evaluated with qPCR, while N. ceranae infection was measured by spore counts. N. ceranae infection level was significantly higher in comparison with that of L. passim (spore or cell equivalents/bee, respectively). Significant positive correlation between infection levels of the parasite species indicates their similar annual dynamics, whilst the differences in the levels of infection between particular months point to a seasonal pattern in the incidence of both parasites. The assay which has been developed and validated creates opportunity for detailed study of L. passim infection kinetics and the improvement in the management practices in beekeeping related to these two parasites.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Invertebrate Pathology
dc.subjectApis melliferaen
dc.subjectCo-infectionen
dc.subjectInterspecific dynamicen
dc.subjectLotmaria passimen
dc.subjectNosema ceranaeen
dc.subjectqPCRen
dc.titleQuantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranaeen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈовановић, Немања, М.; Вејновић, Бранислав; Станимировић, Зоран; Сцхwарз, Рyан С.; Мириловић, Милорад; Aлексић, Невенка; Стевановић, Јевросима;
dc.citation.volume151
dc.citation.spage76
dc.citation.epage81
dc.citation.other151: 76-81
dc.citation.rankM21
dc.identifier.wos000424857300009
dc.identifier.doi10.1016/j.jip.2017.11.003
dc.identifier.pmid29113738
dc.identifier.scopus2-s2.0-85033564300
dc.type.versionpublishedVersion


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