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dc.creatorTodorović, Dalibor
dc.creatorVelhner, Maja
dc.creatorGrego, Edita
dc.creatorVidanović, Dejan
dc.creatorMilanov, Dubravka
dc.creatorKrnjaić, Dejan
dc.creatorKehrenberg, Corinna
dc.date.accessioned2020-06-03T14:27:00Z
dc.date.available2020-06-03T14:27:00Z
dc.date.issued2018
dc.identifier.issn1076-6294
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1648
dc.description.abstractThe aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.en
dc.publisherMary Ann Liebert, Inc, New Rochelle
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31071/RS//
dc.rightsrestrictedAccess
dc.sourceMicrobial Drug Resistance
dc.subjectEen
dc.subjectcolien
dc.subjectpigsen
dc.subjectcow milken
dc.subjectPFGEen
dc.subjectresistance genesen
dc.subjectintegronsen
dc.subjectconjugative plasmidsen
dc.titleMolecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbiaen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВидановић, Дејан; Кехренберг, Цоринна; Грего, Едита; Миланов, Дубравка; Тодоровић, Далибор; Велхнер, Маја; Крњаић, Дејан;
dc.citation.volume24
dc.citation.issue1
dc.citation.spage95
dc.citation.epage103
dc.citation.other24(1): 95-103
dc.citation.rankM22
dc.identifier.wos000419819100015
dc.identifier.doi10.1089/mdr.2017.0016
dc.identifier.pmid28520501
dc.identifier.scopus2-s2.0-85040590273
dc.identifier.rcubconv_2339
dc.type.versionpublishedVersion


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