Determination of the Fatty Acids in Fish Tissue and Feed - Comparison of Different Methods and Statistical Evaluation
2018
Authors
Trbović, DejanaPolak, T.
Demsar, L.
Parunović, N.
Dimitrijević, Mirjana
Nikolić, Dragica M.
Đorđević, V.
Article (Published version)
Metadata
Show full item recordAbstract
With the aim to reinforce laboratory competence in the field of testing the quality of fish from aquaculture, a study on the precision of fatty acid (FA) analyses in fish meat and fish feed was undertaken. Different methods were performed in laboratories. In situ transesterification method and extraction of lipids from the fish were followed by capillary gas chromatography with flame ionization detection. The reproducibility (R) values of the majority of FM were less than 3% of their absolute values. Differences in calculating ionization detector response factors and/or autoxidation caused by faulty sample-handling could lead to variation in quantification of FAs in fish, especially for FA C22:6n-3. Statistical analysis showed a significant correlation between the two laboratories quantifications of FM in fish and fish feed (Pearsons correlation coefficient; r = 0.987, r = 0.994, and r = 0.997; for fish Z [trout], fish g [rainbow trout], and fish feed, respectively). Overall, adequate ...accuracy was obtained in this study. The proposed method provides a fast and efficient means of identifying fish and feed for quality control purposes.
Keywords:
Fish tissues / fatty acids / quantitation / gas chromatography / reproducibilitySource:
Acta Chromatographica, 2018, 30, 3, 175-179Publisher:
- Akademiai Kiado Zrt, Budapest
Funding / projects:
- Bilateral project - Ministry of Education, Science and Technological Development of the Republic of Serbia [405-03-3095/2014-09/49]
- Ministry of Higher Education, Science and Technology of the Republic of Slovenia [BI-RS/14-15-058]
DOI: 10.1556/1326.2017.00165
ISSN: 1233-2356
WoS: 000443278200006
Scopus: 2-s2.0-85052898453
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Trbović, Dejana AU - Polak, T. AU - Demsar, L. AU - Parunović, N. AU - Dimitrijević, Mirjana AU - Nikolić, Dragica M. AU - Đorđević, V. PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1663 AB - With the aim to reinforce laboratory competence in the field of testing the quality of fish from aquaculture, a study on the precision of fatty acid (FA) analyses in fish meat and fish feed was undertaken. Different methods were performed in laboratories. In situ transesterification method and extraction of lipids from the fish were followed by capillary gas chromatography with flame ionization detection. The reproducibility (R) values of the majority of FM were less than 3% of their absolute values. Differences in calculating ionization detector response factors and/or autoxidation caused by faulty sample-handling could lead to variation in quantification of FAs in fish, especially for FA C22:6n-3. Statistical analysis showed a significant correlation between the two laboratories quantifications of FM in fish and fish feed (Pearsons correlation coefficient; r = 0.987, r = 0.994, and r = 0.997; for fish Z [trout], fish g [rainbow trout], and fish feed, respectively). Overall, adequate accuracy was obtained in this study. The proposed method provides a fast and efficient means of identifying fish and feed for quality control purposes. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Chromatographica T1 - Determination of the Fatty Acids in Fish Tissue and Feed - Comparison of Different Methods and Statistical Evaluation VL - 30 IS - 3 SP - 175 EP - 179 DO - 10.1556/1326.2017.00165 ER -
@article{ author = "Trbović, Dejana and Polak, T. and Demsar, L. and Parunović, N. and Dimitrijević, Mirjana and Nikolić, Dragica M. and Đorđević, V.", year = "2018", abstract = "With the aim to reinforce laboratory competence in the field of testing the quality of fish from aquaculture, a study on the precision of fatty acid (FA) analyses in fish meat and fish feed was undertaken. Different methods were performed in laboratories. In situ transesterification method and extraction of lipids from the fish were followed by capillary gas chromatography with flame ionization detection. The reproducibility (R) values of the majority of FM were less than 3% of their absolute values. Differences in calculating ionization detector response factors and/or autoxidation caused by faulty sample-handling could lead to variation in quantification of FAs in fish, especially for FA C22:6n-3. Statistical analysis showed a significant correlation between the two laboratories quantifications of FM in fish and fish feed (Pearsons correlation coefficient; r = 0.987, r = 0.994, and r = 0.997; for fish Z [trout], fish g [rainbow trout], and fish feed, respectively). Overall, adequate accuracy was obtained in this study. The proposed method provides a fast and efficient means of identifying fish and feed for quality control purposes.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Chromatographica", title = "Determination of the Fatty Acids in Fish Tissue and Feed - Comparison of Different Methods and Statistical Evaluation", volume = "30", number = "3", pages = "175-179", doi = "10.1556/1326.2017.00165" }
Trbović, D., Polak, T., Demsar, L., Parunović, N., Dimitrijević, M., Nikolić, D. M.,& Đorđević, V.. (2018). Determination of the Fatty Acids in Fish Tissue and Feed - Comparison of Different Methods and Statistical Evaluation. in Acta Chromatographica Akademiai Kiado Zrt, Budapest., 30(3), 175-179. https://doi.org/10.1556/1326.2017.00165
Trbović D, Polak T, Demsar L, Parunović N, Dimitrijević M, Nikolić DM, Đorđević V. Determination of the Fatty Acids in Fish Tissue and Feed - Comparison of Different Methods and Statistical Evaluation. in Acta Chromatographica. 2018;30(3):175-179. doi:10.1556/1326.2017.00165 .
Trbović, Dejana, Polak, T., Demsar, L., Parunović, N., Dimitrijević, Mirjana, Nikolić, Dragica M., Đorđević, V., "Determination of the Fatty Acids in Fish Tissue and Feed - Comparison of Different Methods and Statistical Evaluation" in Acta Chromatographica, 30, no. 3 (2018):175-179, https://doi.org/10.1556/1326.2017.00165 . .