Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite
2018
Authors
Marc, SimonaKirovski, Danijela
Mircu, Calin
Hutu, Ioan
Otava, Gabriel
Paul, Cristina
Boldura, Oana Maria
Tulcan, Camelia
Article (Published version)
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The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, alpha 1-globulin, alpha 2-globulin, beta-globulin and gamma-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of gamma-globulins, beta-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance w...ith a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%.
Keywords:
electrophoresis / calves / clinoptilolite / gamma-globulinSource:
Molecules, 2018, 23, 6, 1278-Publisher:
- MDPI, Basel
Funding / projects:
- Interdisciplinary Research Platform and Research Laboratory Center Horia Cernescu - infrastructure project POSCCE SMIS [2669]
DOI: 10.3390/molecules23061278
ISSN: 1420-3049
PubMed: 29861463
WoS: 000435875400040
Scopus: 2-s2.0-85047859316
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Fakultet veterinarske medicineTY - JOUR AU - Marc, Simona AU - Kirovski, Danijela AU - Mircu, Calin AU - Hutu, Ioan AU - Otava, Gabriel AU - Paul, Cristina AU - Boldura, Oana Maria AU - Tulcan, Camelia PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1666 AB - The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, alpha 1-globulin, alpha 2-globulin, beta-globulin and gamma-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of gamma-globulins, beta-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%. PB - MDPI, Basel T2 - Molecules T1 - Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite VL - 23 IS - 6 SP - 1278 DO - 10.3390/molecules23061278 ER -
@article{ author = "Marc, Simona and Kirovski, Danijela and Mircu, Calin and Hutu, Ioan and Otava, Gabriel and Paul, Cristina and Boldura, Oana Maria and Tulcan, Camelia", year = "2018", abstract = "The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, alpha 1-globulin, alpha 2-globulin, beta-globulin and gamma-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of gamma-globulins, beta-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%.", publisher = "MDPI, Basel", journal = "Molecules", title = "Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite", volume = "23", number = "6", pages = "1278", doi = "10.3390/molecules23061278" }
Marc, S., Kirovski, D., Mircu, C., Hutu, I., Otava, G., Paul, C., Boldura, O. M.,& Tulcan, C.. (2018). Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite. in Molecules MDPI, Basel., 23(6), 1278. https://doi.org/10.3390/molecules23061278
Marc S, Kirovski D, Mircu C, Hutu I, Otava G, Paul C, Boldura OM, Tulcan C. Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite. in Molecules. 2018;23(6):1278. doi:10.3390/molecules23061278 .
Marc, Simona, Kirovski, Danijela, Mircu, Calin, Hutu, Ioan, Otava, Gabriel, Paul, Cristina, Boldura, Oana Maria, Tulcan, Camelia, "Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite" in Molecules, 23, no. 6 (2018):1278, https://doi.org/10.3390/molecules23061278 . .