Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail
Abstract
Chlorpyrifos is a widely used organophosphate pesticide (OP). In birds and mammals OP exhibits a toxic effect via inhibition of cholinesterases [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] and through oxidative/nitrosative stress. In this study, the influence of chlorpyrifos on cholinesterase activity, parameters of oxidative stress [malondialdehyde (MDA); glutathione (GSH); superoxide dismutase (SOD); nitrite concentration (NO2-); hydrogen peroxide (H2O2)], and inflammatory parameter [activity of myeloperoxidase (MPO)] in the brain of Japanese quail (Coturnix japanica) was examined. The study was conducted on a total of 60 male Japanese quails (one control and 5 experimental groups, n = 10), 3 to 4 wk old. Quails were administered by gavage chlorpyrifos (CPF) for 7 consecutive da at doses of 0.375 mg/kg BW, 0.75 mg/kg BW, 1.5 mg/kg BW, 3 mg/kg BW, and 6 mg/kg BW. Our studies have shown that all doses of CPF significantly inhibited both cholinesterases in brain: AChE ...from 22.74 to 37.83% and BChE from 19.53 to 61.9%, and that inhibition was dose dependent. Also, CPF has led to an increase in the concentration of MDA, GSH, NO2-, and H2O2 and activity of SOD and MPO. Overall, these results support the hypothesis that CPF causes oxidative stress and inflammatory response. This research was carried out on quails because there is hardly any or not enough data about the neurotoxic effect of CPF and especially about its influence on oxidative stress in birds. This study is highly important because we are witnessing massive avian mortality in certain countries due to pesticides.
Keywords:
chlorpyrifos / Japanese quail / cholinesterase / oxidative stress / brainSource:
Poultry Science, 2018, 97, 5, 1564-1571Publisher:
- Oxford Univ Press, Oxford
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
- Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-MESTD-Basic Research (BR or ON)-173034)
DOI: 10.3382/ps/pey018
ISSN: 0032-5791
PubMed: 29471505
WoS: 000434235300010
Scopus: 2-s2.0-85052438863
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Ćupić Miladinović, Dejana AU - Borozan, Sunčica AU - Ivanović, Saša PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1667 AB - Chlorpyrifos is a widely used organophosphate pesticide (OP). In birds and mammals OP exhibits a toxic effect via inhibition of cholinesterases [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] and through oxidative/nitrosative stress. In this study, the influence of chlorpyrifos on cholinesterase activity, parameters of oxidative stress [malondialdehyde (MDA); glutathione (GSH); superoxide dismutase (SOD); nitrite concentration (NO2-); hydrogen peroxide (H2O2)], and inflammatory parameter [activity of myeloperoxidase (MPO)] in the brain of Japanese quail (Coturnix japanica) was examined. The study was conducted on a total of 60 male Japanese quails (one control and 5 experimental groups, n = 10), 3 to 4 wk old. Quails were administered by gavage chlorpyrifos (CPF) for 7 consecutive da at doses of 0.375 mg/kg BW, 0.75 mg/kg BW, 1.5 mg/kg BW, 3 mg/kg BW, and 6 mg/kg BW. Our studies have shown that all doses of CPF significantly inhibited both cholinesterases in brain: AChE from 22.74 to 37.83% and BChE from 19.53 to 61.9%, and that inhibition was dose dependent. Also, CPF has led to an increase in the concentration of MDA, GSH, NO2-, and H2O2 and activity of SOD and MPO. Overall, these results support the hypothesis that CPF causes oxidative stress and inflammatory response. This research was carried out on quails because there is hardly any or not enough data about the neurotoxic effect of CPF and especially about its influence on oxidative stress in birds. This study is highly important because we are witnessing massive avian mortality in certain countries due to pesticides. PB - Oxford Univ Press, Oxford T2 - Poultry Science T1 - Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail VL - 97 IS - 5 SP - 1564 EP - 1571 DO - 10.3382/ps/pey018 ER -
@article{ author = "Ćupić Miladinović, Dejana and Borozan, Sunčica and Ivanović, Saša", year = "2018", abstract = "Chlorpyrifos is a widely used organophosphate pesticide (OP). In birds and mammals OP exhibits a toxic effect via inhibition of cholinesterases [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] and through oxidative/nitrosative stress. In this study, the influence of chlorpyrifos on cholinesterase activity, parameters of oxidative stress [malondialdehyde (MDA); glutathione (GSH); superoxide dismutase (SOD); nitrite concentration (NO2-); hydrogen peroxide (H2O2)], and inflammatory parameter [activity of myeloperoxidase (MPO)] in the brain of Japanese quail (Coturnix japanica) was examined. The study was conducted on a total of 60 male Japanese quails (one control and 5 experimental groups, n = 10), 3 to 4 wk old. Quails were administered by gavage chlorpyrifos (CPF) for 7 consecutive da at doses of 0.375 mg/kg BW, 0.75 mg/kg BW, 1.5 mg/kg BW, 3 mg/kg BW, and 6 mg/kg BW. Our studies have shown that all doses of CPF significantly inhibited both cholinesterases in brain: AChE from 22.74 to 37.83% and BChE from 19.53 to 61.9%, and that inhibition was dose dependent. Also, CPF has led to an increase in the concentration of MDA, GSH, NO2-, and H2O2 and activity of SOD and MPO. Overall, these results support the hypothesis that CPF causes oxidative stress and inflammatory response. This research was carried out on quails because there is hardly any or not enough data about the neurotoxic effect of CPF and especially about its influence on oxidative stress in birds. This study is highly important because we are witnessing massive avian mortality in certain countries due to pesticides.", publisher = "Oxford Univ Press, Oxford", journal = "Poultry Science", title = "Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail", volume = "97", number = "5", pages = "1564-1571", doi = "10.3382/ps/pey018" }
Ćupić Miladinović, D., Borozan, S.,& Ivanović, S.. (2018). Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail. in Poultry Science Oxford Univ Press, Oxford., 97(5), 1564-1571. https://doi.org/10.3382/ps/pey018
Ćupić Miladinović D, Borozan S, Ivanović S. Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail. in Poultry Science. 2018;97(5):1564-1571. doi:10.3382/ps/pey018 .
Ćupić Miladinović, Dejana, Borozan, Sunčica, Ivanović, Saša, "Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail" in Poultry Science, 97, no. 5 (2018):1564-1571, https://doi.org/10.3382/ps/pey018 . .