Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016
2019
Аутори
Vasić, A.Oșlobanu, L.E.
Marinov, M.
Crivei, L.A.
Rățoi, I.A.
Aniță, A.
Aniță, D.
Doroșencu, A.
Alexe, V.
Raileanu, Cristian
Simeunović, Predrag
Raileanu, Cristian
Falcuța, E.
Prioteasa, F.L.
Bojkovski, Jovan
Pavlović, I.
Mathis, A.
Tews, B.A.
Savuta, Gheorghe
Veronesi, E.
Silaghi, Cornelia
Cimpan, A.
Ciucă, L.
Cojkić, A.
Drašković, Vladimir
Đurić, M.
Glavinić, Uroš
Ivanescu, M.L.
Kavran, M.
Lupu, A.
Mindru, Raluca
Porea, Daniela
Radanović, Oliver
Ristanić, Marko
Roman, Constantin
Stanišić, Ljubodrag
Zdravković, Nemanja
Vaselek, S.
SCOPES, AMSAR training group
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoe...s were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.
Кључне речи:
Aedes spp / Anopheles spp / Danube Delta / ELISA / VNT / West Nile virus / Wild birdsИзвор:
Tropical Medicine and Infectious Disease, 2019, 4, 3, 116-Издавач:
- MDPI AG
Финансирање / пројекти:
- SCOPES program of the Swiss National Science Foundation (SNFS) and the Swiss Agency for Development and Cooperation (SDC).
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Vasić, A. AU - Oșlobanu, L.E. AU - Marinov, M. AU - Crivei, L.A. AU - Rățoi, I.A. AU - Aniță, A. AU - Aniță, D. AU - Doroșencu, A. AU - Alexe, V. AU - Raileanu, Cristian AU - Simeunović, Predrag AU - Raileanu, Cristian AU - Falcuța, E. AU - Prioteasa, F.L. AU - Bojkovski, Jovan AU - Pavlović, I. AU - Mathis, A. AU - Tews, B.A. AU - Savuta, Gheorghe AU - Veronesi, E. AU - Silaghi, Cornelia AU - Cimpan, A. AU - Ciucă, L. AU - Cojkić, A. AU - Drašković, Vladimir AU - Đurić, M. AU - Glavinić, Uroš AU - Ivanescu, M.L. AU - Kavran, M. AU - Lupu, A. AU - Mindru, Raluca AU - Porea, Daniela AU - Radanović, Oliver AU - Ristanić, Marko AU - Roman, Constantin AU - Stanišić, Ljubodrag AU - Zdravković, Nemanja AU - Vaselek, S. AU - SCOPES, AMSAR training group PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1720 AB - West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016. PB - MDPI AG T2 - Tropical Medicine and Infectious Disease T1 - Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016 VL - 4 IS - 3 SP - 116 DO - 10.3390/tropicalmed4030116 ER -
@article{ author = "Vasić, A. and Oșlobanu, L.E. and Marinov, M. and Crivei, L.A. and Rățoi, I.A. and Aniță, A. and Aniță, D. and Doroșencu, A. and Alexe, V. and Raileanu, Cristian and Simeunović, Predrag and Raileanu, Cristian and Falcuța, E. and Prioteasa, F.L. and Bojkovski, Jovan and Pavlović, I. and Mathis, A. and Tews, B.A. and Savuta, Gheorghe and Veronesi, E. and Silaghi, Cornelia and Cimpan, A. and Ciucă, L. and Cojkić, A. and Drašković, Vladimir and Đurić, M. and Glavinić, Uroš and Ivanescu, M.L. and Kavran, M. and Lupu, A. and Mindru, Raluca and Porea, Daniela and Radanović, Oliver and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Zdravković, Nemanja and Vaselek, S. and SCOPES, AMSAR training group", year = "2019", abstract = "West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.", publisher = "MDPI AG", journal = "Tropical Medicine and Infectious Disease", title = "Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016", volume = "4", number = "3", pages = "116", doi = "10.3390/tropicalmed4030116" }
Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, C., Simeunović, P., Raileanu, C., Falcuța, E., Prioteasa, F.L., Bojkovski, J., Pavlović, I., Mathis, A., Tews, B.A., Savuta, G., Veronesi, E., Silaghi, C., Cimpan, A., Ciucă, L., Cojkić, A., Drašković, V., Đurić, M., Glavinić, U., Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, R., Porea, D., Radanović, O., Ristanić, M., Roman, C., Stanišić, L., Zdravković, N., Vaselek, S.,& SCOPES, A. t. g.. (2019). Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease MDPI AG., 4(3), 116. https://doi.org/10.3390/tropicalmed4030116
Vasić A, Oșlobanu L, Marinov M, Crivei L, Rățoi I, Aniță A, Aniță D, Doroșencu A, Alexe V, Raileanu C, Simeunović P, Raileanu C, Falcuța E, Prioteasa F, Bojkovski J, Pavlović I, Mathis A, Tews B, Savuta G, Veronesi E, Silaghi C, Cimpan A, Ciucă L, Cojkić A, Drašković V, Đurić M, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Radanović O, Ristanić M, Roman C, Stanišić L, Zdravković N, Vaselek S, SCOPES ATG. Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease. 2019;4(3):116. doi:10.3390/tropicalmed4030116 .
Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, Cristian, Simeunović, Predrag, Raileanu, Cristian, Falcuța, E., Prioteasa, F.L., Bojkovski, Jovan, Pavlović, I., Mathis, A., Tews, B.A., Savuta, Gheorghe, Veronesi, E., Silaghi, Cornelia, Cimpan, A., Ciucă, L., Cojkić, A., Drašković, Vladimir, Đurić, M., Glavinić, Uroš, Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, Raluca, Porea, Daniela, Radanović, Oliver, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Zdravković, Nemanja, Vaselek, S., SCOPES, AMSAR training group, "Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016" in Tropical Medicine and Infectious Disease, 4, no. 3 (2019):116, https://doi.org/10.3390/tropicalmed4030116 . .