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dc.creatorZuber, Ivana
dc.creatorLakićević, Brankica
dc.creatorPietzka, Ariane
dc.creatorMilanov, Dubravka
dc.creatorĐorđević, Vesna
dc.creatorKarabasil, Neđeljko
dc.creatorTeodorović, Vlado
dc.creatorRuppitsch, Werner
dc.creatorDimitrijević, Mirjana
dc.date.accessioned2020-06-03T14:32:41Z
dc.date.available2020-06-03T14:32:41Z
dc.date.issued2019
dc.identifier.issn0740-0020
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1728
dc.description.abstractThe presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.en
dc.publisherAcademic Press Ltd- Elsevier Science Ltd, London
dc.relationMinistry of Defence, Republic of Serbia [MFVMA/1/17-19]
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31034/RS//
dc.rightsrestrictedAccess
dc.sourceFood Microbiology
dc.subjectListeria monocytogenesen
dc.subjectMeat productsen
dc.subjectcgMLSTen
dc.subjectAMRen
dc.subjectDisinfectant resistanceen
dc.titleMolecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДимитријевић, Мирјана; Руппитсцх, Wернер; Миланов, Дубравка; Теодоровић, Владо; Лакићевић, Бранкица; Ђорђевић, Весна; Карабасил, Неђељко; Пиетзка, Aриане; Зубер, Ивана;
dc.citation.volume79
dc.citation.spage116
dc.citation.epage122
dc.citation.other79: 116-122
dc.citation.rankaM21
dc.description.otherPeer-reviewed manuscript: [http://vet-erinar.vet.bg.ac.rs/handle/123456789/1844]
dc.identifier.wos000454886100014
dc.identifier.doi10.1016/j.fm.2018.12.005
dc.identifier.pmid30621866
dc.identifier.scopus2-s2.0-85058799684
dc.identifier.rcubconv_2450
dc.type.versionpublishedVersion


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