Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia
Само за регистроване кориснике
2019
Аутори
Milojević, LazarVelebit, Branko
Teodorović, Vlado
Kirbis, Andrej
Petrović, Tamaš
Karabasil, Nedjeljko
Dimitrijević, Mirjana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Hepatitis E virus (HEV) is a zoonotic virus that can cause acute hepatitis in humans. Besides the fecal-oral route, transmission can occur by consumption of undercooked pig liver. Genotype 3 is the most frequent genotype found in Europe. Studies on HEV in slaughter-age pigs have not been conducted in Serbia so far. Pork meat production and consumption in Serbia is on average, higher than in the rest of Europe. With the aim to identify the circulating HEV genotypes, pig livers and swab samples from three pig slaughterhouses located in three different sub-regions of Serbia were collected. A nested RT-PCR was used to amplify the hypervariable HEV ORF-1 region (334 bp). The amplicons yielded in this study were sequenced, and a molecular phylogeny analysis based on the maximum likelihood method, including HEV sequences reported in several other countries, was performed. The average prevalence of HEV genotype 3 in 3-month-old pigs was 34%. Phylogenetic analysis revealed the majority of HEV a...mplification fragments from Serbia were grouped in four clades within sub-genotype 3a and were also genetically related to German, Italian, Slovenian, and American HEV sequences. Sub-genotypes 3b and 3j were also found in a single pig each. This study provides the first analysis of the genetic diversity and circulation dynamics of HEV in pigs at slaughterhouses in Serbia.
Кључне речи:
Hepatitis E virus / Slaughterhouse / Pigs / SerbiaИзвор:
Food and Environmental Virology, 2019, 11, 4, 410-419Издавач:
- Springer, New York
Финансирање / пројекти:
- Serbian Ministry of Education, Science and Technological Development
DOI: 10.1007/s12560-019-09393-1
ISSN: 1867-0334
PubMed: 31243738
WoS: 000495795000008
Scopus: 2-s2.0-85068319787
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Milojević, Lazar AU - Velebit, Branko AU - Teodorović, Vlado AU - Kirbis, Andrej AU - Petrović, Tamaš AU - Karabasil, Nedjeljko AU - Dimitrijević, Mirjana PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1766 AB - Hepatitis E virus (HEV) is a zoonotic virus that can cause acute hepatitis in humans. Besides the fecal-oral route, transmission can occur by consumption of undercooked pig liver. Genotype 3 is the most frequent genotype found in Europe. Studies on HEV in slaughter-age pigs have not been conducted in Serbia so far. Pork meat production and consumption in Serbia is on average, higher than in the rest of Europe. With the aim to identify the circulating HEV genotypes, pig livers and swab samples from three pig slaughterhouses located in three different sub-regions of Serbia were collected. A nested RT-PCR was used to amplify the hypervariable HEV ORF-1 region (334 bp). The amplicons yielded in this study were sequenced, and a molecular phylogeny analysis based on the maximum likelihood method, including HEV sequences reported in several other countries, was performed. The average prevalence of HEV genotype 3 in 3-month-old pigs was 34%. Phylogenetic analysis revealed the majority of HEV amplification fragments from Serbia were grouped in four clades within sub-genotype 3a and were also genetically related to German, Italian, Slovenian, and American HEV sequences. Sub-genotypes 3b and 3j were also found in a single pig each. This study provides the first analysis of the genetic diversity and circulation dynamics of HEV in pigs at slaughterhouses in Serbia. PB - Springer, New York T2 - Food and Environmental Virology T1 - Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia VL - 11 IS - 4 SP - 410 EP - 419 DO - 10.1007/s12560-019-09393-1 ER -
@article{ author = "Milojević, Lazar and Velebit, Branko and Teodorović, Vlado and Kirbis, Andrej and Petrović, Tamaš and Karabasil, Nedjeljko and Dimitrijević, Mirjana", year = "2019", abstract = "Hepatitis E virus (HEV) is a zoonotic virus that can cause acute hepatitis in humans. Besides the fecal-oral route, transmission can occur by consumption of undercooked pig liver. Genotype 3 is the most frequent genotype found in Europe. Studies on HEV in slaughter-age pigs have not been conducted in Serbia so far. Pork meat production and consumption in Serbia is on average, higher than in the rest of Europe. With the aim to identify the circulating HEV genotypes, pig livers and swab samples from three pig slaughterhouses located in three different sub-regions of Serbia were collected. A nested RT-PCR was used to amplify the hypervariable HEV ORF-1 region (334 bp). The amplicons yielded in this study were sequenced, and a molecular phylogeny analysis based on the maximum likelihood method, including HEV sequences reported in several other countries, was performed. The average prevalence of HEV genotype 3 in 3-month-old pigs was 34%. Phylogenetic analysis revealed the majority of HEV amplification fragments from Serbia were grouped in four clades within sub-genotype 3a and were also genetically related to German, Italian, Slovenian, and American HEV sequences. Sub-genotypes 3b and 3j were also found in a single pig each. This study provides the first analysis of the genetic diversity and circulation dynamics of HEV in pigs at slaughterhouses in Serbia.", publisher = "Springer, New York", journal = "Food and Environmental Virology", title = "Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia", volume = "11", number = "4", pages = "410-419", doi = "10.1007/s12560-019-09393-1" }
Milojević, L., Velebit, B., Teodorović, V., Kirbis, A., Petrović, T., Karabasil, N.,& Dimitrijević, M.. (2019). Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia. in Food and Environmental Virology Springer, New York., 11(4), 410-419. https://doi.org/10.1007/s12560-019-09393-1
Milojević L, Velebit B, Teodorović V, Kirbis A, Petrović T, Karabasil N, Dimitrijević M. Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia. in Food and Environmental Virology. 2019;11(4):410-419. doi:10.1007/s12560-019-09393-1 .
Milojević, Lazar, Velebit, Branko, Teodorović, Vlado, Kirbis, Andrej, Petrović, Tamaš, Karabasil, Nedjeljko, Dimitrijević, Mirjana, "Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia" in Food and Environmental Virology, 11, no. 4 (2019):410-419, https://doi.org/10.1007/s12560-019-09393-1 . .