Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia
2019
Authors
Bozic (Durdevic), BiljanaVučićević, Ivana
Polaček, Vladimir
Vasković, Nikola
Petrović, Tamaš
Pajić, Marko
Aleksić-Kovačević, Sanja
Article (Published version)
Metadata
Show full item recordAbstract
The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and 2016-2017. Avian influenza virus subtype H5N8 isolated from mute swans in 2016-2017 was clustered within the 2.3.4.4 Glade group B. After necropsy, lung, liver, spleen, pancreas, kidney and brain tissues were sampled for histopathology and immunohistochemical examination. Avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most significant gross lesions were necrosis and haemorrhages in the pancreas. Major histological lesions were multifocal necroses in the pancreas, spleen and liver, non-purulent encephalitis, lung congestion and oedema. Immunohistochemical demons...tration of HPAIV nucleoprotein in pancreas and brain was strongly consistent with histological lesions in both infected groups. Our findings showed that pancreas was the most affected organ in all examined mute swans. In addition to increased mortality rate, similar pathological findings were detected in mute swans naturally infected with highly pathogenic avian influenza viruses H5N1 and H5N8.
Keywords:
H5N1 / H5N8 / Pathological lesions / Mute swans / Pancreas / SerbiaSource:
Veterinaria Italiana, 2019, 55, 1, 95-101Publisher:
- Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo
Funding / projects:
- The influence of the quality of the components of food for cyprinid fish species on the quality of meat, losses and the profitability of production (RS-31011)
- Wild animal health monitoring and introduction of new biotechnology procedures in detection of infectious and zoonotic agents - risk analysis for human health, domestic and wild animal health and for environmental contamination (RS-31084)
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-46002)
DOI: 10.12834/VetIt.1463.7919.2
ISSN: 0505-401X
PubMed: 30951187
WoS: 000496972400011
Scopus: 2-s2.0-85064313511
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Bozic (Durdevic), Biljana AU - Vučićević, Ivana AU - Polaček, Vladimir AU - Vasković, Nikola AU - Petrović, Tamaš AU - Pajić, Marko AU - Aleksić-Kovačević, Sanja PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1770 AB - The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and 2016-2017. Avian influenza virus subtype H5N8 isolated from mute swans in 2016-2017 was clustered within the 2.3.4.4 Glade group B. After necropsy, lung, liver, spleen, pancreas, kidney and brain tissues were sampled for histopathology and immunohistochemical examination. Avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most significant gross lesions were necrosis and haemorrhages in the pancreas. Major histological lesions were multifocal necroses in the pancreas, spleen and liver, non-purulent encephalitis, lung congestion and oedema. Immunohistochemical demonstration of HPAIV nucleoprotein in pancreas and brain was strongly consistent with histological lesions in both infected groups. Our findings showed that pancreas was the most affected organ in all examined mute swans. In addition to increased mortality rate, similar pathological findings were detected in mute swans naturally infected with highly pathogenic avian influenza viruses H5N1 and H5N8. PB - Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo T2 - Veterinaria Italiana T1 - Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia VL - 55 IS - 1 SP - 95 EP - 101 DO - 10.12834/VetIt.1463.7919.2 ER -
@article{ author = "Bozic (Durdevic), Biljana and Vučićević, Ivana and Polaček, Vladimir and Vasković, Nikola and Petrović, Tamaš and Pajić, Marko and Aleksić-Kovačević, Sanja", year = "2019", abstract = "The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and 2016-2017. Avian influenza virus subtype H5N8 isolated from mute swans in 2016-2017 was clustered within the 2.3.4.4 Glade group B. After necropsy, lung, liver, spleen, pancreas, kidney and brain tissues were sampled for histopathology and immunohistochemical examination. Avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most significant gross lesions were necrosis and haemorrhages in the pancreas. Major histological lesions were multifocal necroses in the pancreas, spleen and liver, non-purulent encephalitis, lung congestion and oedema. Immunohistochemical demonstration of HPAIV nucleoprotein in pancreas and brain was strongly consistent with histological lesions in both infected groups. Our findings showed that pancreas was the most affected organ in all examined mute swans. In addition to increased mortality rate, similar pathological findings were detected in mute swans naturally infected with highly pathogenic avian influenza viruses H5N1 and H5N8.", publisher = "Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo", journal = "Veterinaria Italiana", title = "Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia", volume = "55", number = "1", pages = "95-101", doi = "10.12834/VetIt.1463.7919.2" }
Bozic (Durdevic), B., Vučićević, I., Polaček, V., Vasković, N., Petrović, T., Pajić, M.,& Aleksić-Kovačević, S.. (2019). Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia. in Veterinaria Italiana Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo., 55(1), 95-101. https://doi.org/10.12834/VetIt.1463.7919.2
Bozic (Durdevic) B, Vučićević I, Polaček V, Vasković N, Petrović T, Pajić M, Aleksić-Kovačević S. Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia. in Veterinaria Italiana. 2019;55(1):95-101. doi:10.12834/VetIt.1463.7919.2 .
Bozic (Durdevic), Biljana, Vučićević, Ivana, Polaček, Vladimir, Vasković, Nikola, Petrović, Tamaš, Pajić, Marko, Aleksić-Kovačević, Sanja, "Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia" in Veterinaria Italiana, 55, no. 1 (2019):95-101, https://doi.org/10.12834/VetIt.1463.7919.2 . .