Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs
2019
Autori
Milanović, ZoranaVekić, Jelena
Radonjić, Vladimir
Božović-Ilić, Anja
Zeljković, Aleksandra
Janac, Jelena
Spasojevic-Kalimanovska, Vesna
Buch, Jesse
Chandrashekar, Ramaswamy
Bojic-Trbojević, Zanka
Hajduković, Ljiljana
Christopher, Mary M.
Kovačević-Filipović, Milica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had signi...ficantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.
Ključne reči:
acute phase response / apolipoprotein A-1 / high-density lipoprotein / lipoprotein diameter / serum amyloid AIzvor:
Journal of Veterinary Internal Medicine, 2019, 33, 4, 1686-1694Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- IDEXX Laboratories, Inc., Westbrook, Maine, USA
- LKB Vertriebs Ges.m.b.h., Belgrade
- Interaktivna uloga dislipidemije, oksidativnog stresa i inflamacije u aterosklerozi i drugim bolestima: genetički i biohemijski markeri (RS-175035)
- Antioksidativna zaštita i potencijali za diferencijaciju i regeneraciju mezenhimalnih matičnih ćelija iz različitih tkiva tokom procesa starenja (RS-175061)
DOI: 10.1111/jvim.15537
ISSN: 0891-6640
PubMed: 31175698
WoS: 000481436400016
Scopus: 2-s2.0-85067417594
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Milanović, Zorana AU - Vekić, Jelena AU - Radonjić, Vladimir AU - Božović-Ilić, Anja AU - Zeljković, Aleksandra AU - Janac, Jelena AU - Spasojevic-Kalimanovska, Vesna AU - Buch, Jesse AU - Chandrashekar, Ramaswamy AU - Bojic-Trbojević, Zanka AU - Hajduković, Ljiljana AU - Christopher, Mary M. AU - Kovačević-Filipović, Milica PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1778 AB - Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding. PB - Wiley, Hoboken T2 - Journal of Veterinary Internal Medicine T1 - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs VL - 33 IS - 4 SP - 1686 EP - 1694 DO - 10.1111/jvim.15537 ER -
@article{ author = "Milanović, Zorana and Vekić, Jelena and Radonjić, Vladimir and Božović-Ilić, Anja and Zeljković, Aleksandra and Janac, Jelena and Spasojevic-Kalimanovska, Vesna and Buch, Jesse and Chandrashekar, Ramaswamy and Bojic-Trbojević, Zanka and Hajduković, Ljiljana and Christopher, Mary M. and Kovačević-Filipović, Milica", year = "2019", abstract = "Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.", publisher = "Wiley, Hoboken", journal = "Journal of Veterinary Internal Medicine", title = "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs", volume = "33", number = "4", pages = "1686-1694", doi = "10.1111/jvim.15537" }
Milanović, Z., Vekić, J., Radonjić, V., Božović-Ilić, A., Zeljković, A., Janac, J., Spasojevic-Kalimanovska, V., Buch, J., Chandrashekar, R., Bojic-Trbojević, Z., Hajduković, L., Christopher, M. M.,& Kovačević-Filipović, M.. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine Wiley, Hoboken., 33(4), 1686-1694. https://doi.org/10.1111/jvim.15537
Milanović Z, Vekić J, Radonjić V, Božović-Ilić A, Zeljković A, Janac J, Spasojevic-Kalimanovska V, Buch J, Chandrashekar R, Bojic-Trbojević Z, Hajduković L, Christopher MM, Kovačević-Filipović M. Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine. 2019;33(4):1686-1694. doi:10.1111/jvim.15537 .
Milanović, Zorana, Vekić, Jelena, Radonjić, Vladimir, Božović-Ilić, Anja, Zeljković, Aleksandra, Janac, Jelena, Spasojevic-Kalimanovska, Vesna, Buch, Jesse, Chandrashekar, Ramaswamy, Bojic-Trbojević, Zanka, Hajduković, Ljiljana, Christopher, Mary M., Kovačević-Filipović, Milica, "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs" in Journal of Veterinary Internal Medicine, 33, no. 4 (2019):1686-1694, https://doi.org/10.1111/jvim.15537 . .