Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia
2019
Authors
Milovanović, MilovanMilićević, Vesna
Valčić, Miroslav
Stević, Nataša
Nišavić, Jakov
Radojičić, Sonja
Article (Published version)
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Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with vir...uses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.
Keywords:
Isolation / ORFV / PCR / Phylogenetic analysis / SheepSource:
Pakistan Veterinary Journal, 2019, 39, 3, 433-437Publisher:
- Univ Agriculture, Fac Veterinary Science, Faisalabad
Funding / projects:
- Bacterial zoonoses-Development of molecular and immunological diagnostic methods and their standardization (RS-31088)
- Ecological and viral investigation on the presents of emerging zoonoses in national parks in republic of Serbia (RS-37015)
DOI: 10.29261/pakvetj/2019.034
ISSN: 0253-8318
WoS: 000483997500020
Scopus: 2-s2.0-85076203244
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Fakultet veterinarske medicineTY - JOUR AU - Milovanović, Milovan AU - Milićević, Vesna AU - Valčić, Miroslav AU - Stević, Nataša AU - Nišavić, Jakov AU - Radojičić, Sonja PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1781 AB - Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia. PB - Univ Agriculture, Fac Veterinary Science, Faisalabad T2 - Pakistan Veterinary Journal T1 - Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia VL - 39 IS - 3 SP - 433 EP - 437 DO - 10.29261/pakvetj/2019.034 ER -
@article{ author = "Milovanović, Milovan and Milićević, Vesna and Valčić, Miroslav and Stević, Nataša and Nišavić, Jakov and Radojičić, Sonja", year = "2019", abstract = "Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.", publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad", journal = "Pakistan Veterinary Journal", title = "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia", volume = "39", number = "3", pages = "433-437", doi = "10.29261/pakvetj/2019.034" }
Milovanović, M., Milićević, V., Valčić, M., Stević, N., Nišavić, J.,& Radojičić, S.. (2019). Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal Univ Agriculture, Fac Veterinary Science, Faisalabad., 39(3), 433-437. https://doi.org/10.29261/pakvetj/2019.034
Milovanović M, Milićević V, Valčić M, Stević N, Nišavić J, Radojičić S. Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal. 2019;39(3):433-437. doi:10.29261/pakvetj/2019.034 .
Milovanović, Milovan, Milićević, Vesna, Valčić, Miroslav, Stević, Nataša, Nišavić, Jakov, Radojičić, Sonja, "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia" in Pakistan Veterinary Journal, 39, no. 3 (2019):433-437, https://doi.org/10.29261/pakvetj/2019.034 . .