Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus
Само за регистроване кориснике
2019
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with < 1000 CFU/mL of S. aureus or SCM2 group (n = 55) with >= 1000 CFU/mL of causative agent in milk. Significantly lower serum concentrations of high density lipoprotein-cholesterol (HDL-C) in SCM2 group and higher low density lipoprotein-cholesterol (LDL-C) in both SCM groups were recorded. Significantly lower paraoxonase-1 (PON1) activity and higher lipid hydroperoxides (LOOH) concentration in blood and milk were recorded in both groups of SCM cows vs control. In blood serum of cows from SCM2 group significantly higher concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), bu...t significantly lower total antioxidative capacity (TAC) were obtained. In milk serum of SCM cows were detected significantly higher concentrations of H2O2 and MDA, but significantly lower TAC vs control. There was a significant positive correlation between PON1 in serum with HDL-C, but negative correlation with LDL-C and LOOH. In milk, PON1 negatively correlated with LOOH and somatic cell counts (SCC). Strong positive correlation was obtained between PON1 in blood and milk. Oxidative stress and inflammatory reaction induced by SCM significantly lowered PON1 activity in blood and milk of affected cows.
Кључне речи:
Lipid profile / Oxidative stress / PON1 activity in milk / Staphylococcus aureus / CowsИзвор:
Research in Veterinary Science, 2019, 125, 323-332Издавач:
- Elsevier Sci Ltd, Oxford
DOI: 10.1016/j.rvsc.2019.07.016
ISSN: 0034-5288
PubMed: 31352281
WoS: 000484645700046
Scopus: 2-s2.0-85069697015
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Nedić, Svetlana AU - Vakanjac, Slobodanka AU - Samardzija, Marko AU - Borozan, Sunčica PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1782 AB - The aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with < 1000 CFU/mL of S. aureus or SCM2 group (n = 55) with >= 1000 CFU/mL of causative agent in milk. Significantly lower serum concentrations of high density lipoprotein-cholesterol (HDL-C) in SCM2 group and higher low density lipoprotein-cholesterol (LDL-C) in both SCM groups were recorded. Significantly lower paraoxonase-1 (PON1) activity and higher lipid hydroperoxides (LOOH) concentration in blood and milk were recorded in both groups of SCM cows vs control. In blood serum of cows from SCM2 group significantly higher concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), but significantly lower total antioxidative capacity (TAC) were obtained. In milk serum of SCM cows were detected significantly higher concentrations of H2O2 and MDA, but significantly lower TAC vs control. There was a significant positive correlation between PON1 in serum with HDL-C, but negative correlation with LDL-C and LOOH. In milk, PON1 negatively correlated with LOOH and somatic cell counts (SCC). Strong positive correlation was obtained between PON1 in blood and milk. Oxidative stress and inflammatory reaction induced by SCM significantly lowered PON1 activity in blood and milk of affected cows. PB - Elsevier Sci Ltd, Oxford T2 - Research in Veterinary Science T1 - Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus VL - 125 SP - 323 EP - 332 DO - 10.1016/j.rvsc.2019.07.016 ER -
@article{ author = "Nedić, Svetlana and Vakanjac, Slobodanka and Samardzija, Marko and Borozan, Sunčica", year = "2019", abstract = "The aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with < 1000 CFU/mL of S. aureus or SCM2 group (n = 55) with >= 1000 CFU/mL of causative agent in milk. Significantly lower serum concentrations of high density lipoprotein-cholesterol (HDL-C) in SCM2 group and higher low density lipoprotein-cholesterol (LDL-C) in both SCM groups were recorded. Significantly lower paraoxonase-1 (PON1) activity and higher lipid hydroperoxides (LOOH) concentration in blood and milk were recorded in both groups of SCM cows vs control. In blood serum of cows from SCM2 group significantly higher concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), but significantly lower total antioxidative capacity (TAC) were obtained. In milk serum of SCM cows were detected significantly higher concentrations of H2O2 and MDA, but significantly lower TAC vs control. There was a significant positive correlation between PON1 in serum with HDL-C, but negative correlation with LDL-C and LOOH. In milk, PON1 negatively correlated with LOOH and somatic cell counts (SCC). Strong positive correlation was obtained between PON1 in blood and milk. Oxidative stress and inflammatory reaction induced by SCM significantly lowered PON1 activity in blood and milk of affected cows.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Research in Veterinary Science", title = "Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus", volume = "125", pages = "323-332", doi = "10.1016/j.rvsc.2019.07.016" }
Nedić, S., Vakanjac, S., Samardzija, M.,& Borozan, S.. (2019). Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus. in Research in Veterinary Science Elsevier Sci Ltd, Oxford., 125, 323-332. https://doi.org/10.1016/j.rvsc.2019.07.016
Nedić S, Vakanjac S, Samardzija M, Borozan S. Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus. in Research in Veterinary Science. 2019;125:323-332. doi:10.1016/j.rvsc.2019.07.016 .
Nedić, Svetlana, Vakanjac, Slobodanka, Samardzija, Marko, Borozan, Sunčica, "Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus" in Research in Veterinary Science, 125 (2019):323-332, https://doi.org/10.1016/j.rvsc.2019.07.016 . .