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Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties
dc.creator | Kosanović, Maja | |
dc.creator | Cvetković, Jelena | |
dc.creator | Gruden-Movsesijan, Alisa | |
dc.creator | Vasilev, Saša | |
dc.creator | Milanović, Svetlana | |
dc.creator | Ilić, Natasa | |
dc.creator | Sofronic-Milosavljević, Ljiljana | |
dc.date.accessioned | 2020-06-03T14:36:46Z | |
dc.date.available | 2020-06-03T14:36:46Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0141-9838 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/1785 | |
dc.description.abstract | Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173047/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175102/RS// | |
dc.rights | restrictedAccess | |
dc.source | Parasite Immunology | |
dc.subject | ES L1 | en |
dc.subject | exosomes | en |
dc.subject | Helminths | en |
dc.subject | immunomodulation | en |
dc.subject | muscle larvae | en |
dc.subject | nematode | en |
dc.title | Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Софрониц-Милосављевић, Љиљана; Милановић, Светлана; Василев, Саша; Цветковић, Јелена; Илић, Натаса; Косановић, Маја; Груден-Мовсесијан, Aлиса; | |
dc.citation.volume | 41 | |
dc.citation.issue | 10 | |
dc.citation.spage | e12665 | |
dc.citation.other | 41(10): e12665 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000485468400001 | |
dc.identifier.doi | 10.1111/pim.12665 | |
dc.identifier.pmid | 31356691 | |
dc.identifier.scopus | 2-s2.0-85072234758 | |
dc.type.version | publishedVersion |