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dc.creatorKosanović, Maja
dc.creatorCvetković, Jelena
dc.creatorGruden-Movsesijan, Alisa
dc.creatorVasilev, Saša
dc.creatorMilanović, Svetlana
dc.creatorIlić, Natasa
dc.creatorSofronic-Milosavljević, Ljiljana
dc.date.accessioned2020-06-03T14:36:46Z
dc.date.available2020-06-03T14:36:46Z
dc.date.issued2019
dc.identifier.issn0141-9838
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1785
dc.description.abstractAims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173047/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175102/RS//
dc.rightsrestrictedAccess
dc.sourceParasite Immunology
dc.subjectES L1en
dc.subjectexosomesen
dc.subjectHelminthsen
dc.subjectimmunomodulationen
dc.subjectmuscle larvaeen
dc.subjectnematodeen
dc.titleTrichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory propertiesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСофрониц-Милосављевић, Љиљана; Милановић, Светлана; Василев, Саша; Цветковић, Јелена; Илић, Натаса; Косановић, Маја; Груден-Мовсесијан, Aлиса;
dc.citation.volume41
dc.citation.issue10
dc.citation.spagee12665
dc.citation.other41(10): e12665
dc.citation.rankM21
dc.identifier.wos000485468400001
dc.identifier.doi10.1111/pim.12665
dc.identifier.pmid31356691
dc.identifier.scopus2-s2.0-85072234758
dc.identifier.rcubconv_2508
dc.type.versionpublishedVersion


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