Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay
Само за регистроване кориснике
2019
Аутори
Montag, GraciaBankoglu, Ezgi Eylul
Bolte, Annika
Hintzsche, Henning
Đelić, Ninoslav
Stopper, Helga
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation. A 4-hour MMS treatment induced a significant and concentration-dependent increase in DNA damage for both differentiated and undifferentiated cells, but the difference in sensitivity was only significant at the highest concentration. A 4-hour doxorubicin treatment did not induce DNA damage in differentiated HL60 cells, while it did in undifferentiated cells with its highest tested concentration. A one-hour etoposide treatment caused significant increase in DNA damage concentration dependently in both cell variants. This DNA damage was significantly higher in undifferentiated HL60 cells with several tested concentrations of etoposide. The treatment with ...the oxidizing substances hydrogen peroxide and potassium bromate yielded significant DNA damage induction in both undifferentiated and differentiated cells with no difference according to the differentiation status. Doxorubicin and etoposide are known to inhibit topoisomerase II. The activity of this enzyme has been shown to be higher in undifferentiated actively proliferating cells than in differentiated cells. This may be of relevance when exposures to topoisomerase-inhibiting compounds or the genotoxicity of compounds with unknown mechanism of action are assessed in routine testing.
Кључне речи:
Comet assay / HL60 cell / Cell differentiation / GenotoxicityИзвор:
Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 2019, 845, UNSP 402972-Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- hCOMET COST actionEuropean Cooperation in Science and Technology (COST) [15132]
- DAAD through the bilateral PPP-project between Germany and Serbia
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-46002)
DOI: 10.1016/j.mrgentox.2018.10.004
ISSN: 1383-5718
PubMed: 31561892
WoS: 000489193400012
Scopus: 2-s2.0-85055642630
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Montag, Gracia AU - Bankoglu, Ezgi Eylul AU - Bolte, Annika AU - Hintzsche, Henning AU - Đelić, Ninoslav AU - Stopper, Helga PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1789 AB - The aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation. A 4-hour MMS treatment induced a significant and concentration-dependent increase in DNA damage for both differentiated and undifferentiated cells, but the difference in sensitivity was only significant at the highest concentration. A 4-hour doxorubicin treatment did not induce DNA damage in differentiated HL60 cells, while it did in undifferentiated cells with its highest tested concentration. A one-hour etoposide treatment caused significant increase in DNA damage concentration dependently in both cell variants. This DNA damage was significantly higher in undifferentiated HL60 cells with several tested concentrations of etoposide. The treatment with the oxidizing substances hydrogen peroxide and potassium bromate yielded significant DNA damage induction in both undifferentiated and differentiated cells with no difference according to the differentiation status. Doxorubicin and etoposide are known to inhibit topoisomerase II. The activity of this enzyme has been shown to be higher in undifferentiated actively proliferating cells than in differentiated cells. This may be of relevance when exposures to topoisomerase-inhibiting compounds or the genotoxicity of compounds with unknown mechanism of action are assessed in routine testing. PB - Elsevier, Amsterdam T2 - Mutation Research-Genetic Toxicology and Environmental Mutagenesis T1 - Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay VL - 845 SP - UNSP 402972 DO - 10.1016/j.mrgentox.2018.10.004 ER -
@article{ author = "Montag, Gracia and Bankoglu, Ezgi Eylul and Bolte, Annika and Hintzsche, Henning and Đelić, Ninoslav and Stopper, Helga", year = "2019", abstract = "The aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation. A 4-hour MMS treatment induced a significant and concentration-dependent increase in DNA damage for both differentiated and undifferentiated cells, but the difference in sensitivity was only significant at the highest concentration. A 4-hour doxorubicin treatment did not induce DNA damage in differentiated HL60 cells, while it did in undifferentiated cells with its highest tested concentration. A one-hour etoposide treatment caused significant increase in DNA damage concentration dependently in both cell variants. This DNA damage was significantly higher in undifferentiated HL60 cells with several tested concentrations of etoposide. The treatment with the oxidizing substances hydrogen peroxide and potassium bromate yielded significant DNA damage induction in both undifferentiated and differentiated cells with no difference according to the differentiation status. Doxorubicin and etoposide are known to inhibit topoisomerase II. The activity of this enzyme has been shown to be higher in undifferentiated actively proliferating cells than in differentiated cells. This may be of relevance when exposures to topoisomerase-inhibiting compounds or the genotoxicity of compounds with unknown mechanism of action are assessed in routine testing.", publisher = "Elsevier, Amsterdam", journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis", title = "Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay", volume = "845", pages = "UNSP 402972", doi = "10.1016/j.mrgentox.2018.10.004" }
Montag, G., Bankoglu, E. E., Bolte, A., Hintzsche, H., Đelić, N.,& Stopper, H.. (2019). Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis Elsevier, Amsterdam., 845, UNSP 402972. https://doi.org/10.1016/j.mrgentox.2018.10.004
Montag G, Bankoglu EE, Bolte A, Hintzsche H, Đelić N, Stopper H. Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;845:UNSP 402972. doi:10.1016/j.mrgentox.2018.10.004 .
Montag, Gracia, Bankoglu, Ezgi Eylul, Bolte, Annika, Hintzsche, Henning, Đelić, Ninoslav, Stopper, Helga, "Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 845 (2019):UNSP 402972, https://doi.org/10.1016/j.mrgentox.2018.10.004 . .