The performance of seven molecular methods for the detection of prrsv
2020
Аутори
Milićević, VesnaKureljušić, Branislav
Veljović, Ljubiša
Valčić, Miroslav
Stević, Nataša
Savić, Božidar
Radojičić, Sonja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.
Кључне речи:
PRRS / RT-PCR / sensitivity / SerbiaИзвор:
Acta Veterinaria-Beograd, 2020, 70, 1, 51-57Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Развој и стандардизација молекуларних и имунских метода у дијагностици бактеријских зооноза (RS-MESTD-Technological Development (TD or TR)-31088)
- Унапређење производних капацитета шарана (Cyprinus carpio L) програмима исхране и селекције (RS-MESTD-Technological Development (TD or TR)-31075)
DOI: 10.2478/acve-2020-0003
ISSN: 0567-8315
WoS: 000525346500003
Scopus: 2-s2.0-85083807923
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Milićević, Vesna AU - Kureljušić, Branislav AU - Veljović, Ljubiša AU - Valčić, Miroslav AU - Stević, Nataša AU - Savić, Božidar AU - Radojičić, Sonja PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1793 AB - Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - The performance of seven molecular methods for the detection of prrsv VL - 70 IS - 1 SP - 51 EP - 57 DO - 10.2478/acve-2020-0003 ER -
@article{ author = "Milićević, Vesna and Kureljušić, Branislav and Veljović, Ljubiša and Valčić, Miroslav and Stević, Nataša and Savić, Božidar and Radojičić, Sonja", year = "2020", abstract = "Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "The performance of seven molecular methods for the detection of prrsv", volume = "70", number = "1", pages = "51-57", doi = "10.2478/acve-2020-0003" }
Milićević, V., Kureljušić, B., Veljović, L., Valčić, M., Stević, N., Savić, B.,& Radojičić, S.. (2020). The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 51-57. https://doi.org/10.2478/acve-2020-0003
Milićević V, Kureljušić B, Veljović L, Valčić M, Stević N, Savić B, Radojičić S. The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd. 2020;70(1):51-57. doi:10.2478/acve-2020-0003 .
Milićević, Vesna, Kureljušić, Branislav, Veljović, Ljubiša, Valčić, Miroslav, Stević, Nataša, Savić, Božidar, Radojičić, Sonja, "The performance of seven molecular methods for the detection of prrsv" in Acta Veterinaria-Beograd, 70, no. 1 (2020):51-57, https://doi.org/10.2478/acve-2020-0003 . .