Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
2020
Authors
Matović, KazimirVidanović, Dejan
Manić, Marija
Stojiljković, Marko
Radojičić, Sonja
Debeljak, Zoran
Šekler, Milanko
Ćirić, Jelena
Article (Published version)
Metadata
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A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.
Keywords:
Apis mellifera / Nosema ceranae / Nosema apis / SerbiaSource:
Saudi Journal of Biological Sciences, 2020, 27, 1, 518-523Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Bacterial zoonoses-Development of molecular and immunological diagnostic methods and their standardization (RS-MESTD-Technological Development (TD or TR)-31088)
DOI: 10.1016/j.sjbs.2019.11.012
ISSN: 1319-562X
PubMed: 31889877
WoS: 000503431500069
Scopus: 2-s2.0-85076587330
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Fakultet veterinarske medicineTY - JOUR AU - Matović, Kazimir AU - Vidanović, Dejan AU - Manić, Marija AU - Stojiljković, Marko AU - Radojičić, Sonja AU - Debeljak, Zoran AU - Šekler, Milanko AU - Ćirić, Jelena PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1797 AB - A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection. PB - Elsevier, Amsterdam T2 - Saudi Journal of Biological Sciences T1 - Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia VL - 27 IS - 1 SP - 518 EP - 523 DO - 10.1016/j.sjbs.2019.11.012 ER -
@article{ author = "Matović, Kazimir and Vidanović, Dejan and Manić, Marija and Stojiljković, Marko and Radojičić, Sonja and Debeljak, Zoran and Šekler, Milanko and Ćirić, Jelena", year = "2020", abstract = "A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.", publisher = "Elsevier, Amsterdam", journal = "Saudi Journal of Biological Sciences", title = "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia", volume = "27", number = "1", pages = "518-523", doi = "10.1016/j.sjbs.2019.11.012" }
Matović, K., Vidanović, D., Manić, M., Stojiljković, M., Radojičić, S., Debeljak, Z., Šekler, M.,& Ćirić, J.. (2020). Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences Elsevier, Amsterdam., 27(1), 518-523. https://doi.org/10.1016/j.sjbs.2019.11.012
Matović K, Vidanović D, Manić M, Stojiljković M, Radojičić S, Debeljak Z, Šekler M, Ćirić J. Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences. 2020;27(1):518-523. doi:10.1016/j.sjbs.2019.11.012 .
Matović, Kazimir, Vidanović, Dejan, Manić, Marija, Stojiljković, Marko, Radojičić, Sonja, Debeljak, Zoran, Šekler, Milanko, Ćirić, Jelena, "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia" in Saudi Journal of Biological Sciences, 27, no. 1 (2020):518-523, https://doi.org/10.1016/j.sjbs.2019.11.012 . .