Analysis of sister-chromatid exchanges and micronuclei in cultured human lymphocytes treated with insulin
Апстракт
Insulin is an anabolic hormone that may facilitate development of malignant diseases in various susceptible tissues due to stimulation of mitotic divisions. In this work, an evaluation of mitogenic and genotoxic effects of human recombinant insulin has been performed in cultures of human peripheral blood lymphocytes. Genotoxic effects were studied by the following test systems: (1) in vitro SCE test, and (2) cytokinesis-blocked micronucleus assay. The obtained results indicate that insulin stimulates mitotic division at an optimal concentration of 10-8 M. On the other hand, insulin has not exhibited genotoxic properties under experimental conditions in this investigation.
Кључне речи:
insulin / lymphocyte / sister-chromatid exchange / micronucleus / mitotic indexИзвор:
Folia Biologica, 2001, 47, 1, 28-31Издавач:
- Charles Univ Prague, First Faculty Medicine, Prague 6
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Đelić, Ninoslav PY - 2001 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/180 AB - Insulin is an anabolic hormone that may facilitate development of malignant diseases in various susceptible tissues due to stimulation of mitotic divisions. In this work, an evaluation of mitogenic and genotoxic effects of human recombinant insulin has been performed in cultures of human peripheral blood lymphocytes. Genotoxic effects were studied by the following test systems: (1) in vitro SCE test, and (2) cytokinesis-blocked micronucleus assay. The obtained results indicate that insulin stimulates mitotic division at an optimal concentration of 10-8 M. On the other hand, insulin has not exhibited genotoxic properties under experimental conditions in this investigation. PB - Charles Univ Prague, First Faculty Medicine, Prague 6 T2 - Folia Biologica T1 - Analysis of sister-chromatid exchanges and micronuclei in cultured human lymphocytes treated with insulin VL - 47 IS - 1 SP - 28 EP - 31 UR - https://hdl.handle.net/21.15107/rcub_veterinar_180 ER -
@article{ author = "Đelić, Ninoslav", year = "2001", abstract = "Insulin is an anabolic hormone that may facilitate development of malignant diseases in various susceptible tissues due to stimulation of mitotic divisions. In this work, an evaluation of mitogenic and genotoxic effects of human recombinant insulin has been performed in cultures of human peripheral blood lymphocytes. Genotoxic effects were studied by the following test systems: (1) in vitro SCE test, and (2) cytokinesis-blocked micronucleus assay. The obtained results indicate that insulin stimulates mitotic division at an optimal concentration of 10-8 M. On the other hand, insulin has not exhibited genotoxic properties under experimental conditions in this investigation.", publisher = "Charles Univ Prague, First Faculty Medicine, Prague 6", journal = "Folia Biologica", title = "Analysis of sister-chromatid exchanges and micronuclei in cultured human lymphocytes treated with insulin", volume = "47", number = "1", pages = "28-31", url = "https://hdl.handle.net/21.15107/rcub_veterinar_180" }
Đelić, N.. (2001). Analysis of sister-chromatid exchanges and micronuclei in cultured human lymphocytes treated with insulin. in Folia Biologica Charles Univ Prague, First Faculty Medicine, Prague 6., 47(1), 28-31. https://hdl.handle.net/21.15107/rcub_veterinar_180
Đelić N. Analysis of sister-chromatid exchanges and micronuclei in cultured human lymphocytes treated with insulin. in Folia Biologica. 2001;47(1):28-31. https://hdl.handle.net/21.15107/rcub_veterinar_180 .
Đelić, Ninoslav, "Analysis of sister-chromatid exchanges and micronuclei in cultured human lymphocytes treated with insulin" in Folia Biologica, 47, no. 1 (2001):28-31, https://hdl.handle.net/21.15107/rcub_veterinar_180 .