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dc.creatorCvetković, Mirjana
dc.creatorDamjanović, Ana
dc.creatorStanojković, Tatjana P.
dc.creatorĐorđević, Iris
dc.creatorTešević, Vele
dc.creatorMilosavljević, Slobodan M.
dc.creatorGođevac, Dejan
dc.date.accessioned2020-06-03T14:37:57Z
dc.date.available2020-06-03T14:37:57Z
dc.date.issued2020
dc.identifier.issn0039-9140
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1802
dc.description.abstractMetabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using thy-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIRbased metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.en
dc.publisherElsevier, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS//
dc.rightsopenAccess
dc.sourceTalanta
dc.subjectAmphoricarpos autariatusen
dc.subjectNMRen
dc.subjectFTIRen
dc.subjectMetabolomicsen
dc.subjectCytotoxic activityen
dc.subjectDry-column flash chromatographyen
dc.titleIntegration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatusen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМилосављевић, Слободан М.; Тешевић, Веле; Цветковић, Мирјана; Ђорђевић, Ирис; Гођевац, Дејан; Дамјановић, Aна; Станојковић, Татјана П.;
dc.citation.volume206
dc.citation.spage120248
dc.citation.other206: 120248
dc.citation.rankM21~
dc.identifier.wos000488416200070
dc.identifier.doi10.1016/j.talanta.2019.120248
dc.identifier.pmid31514863
dc.identifier.scopus2-s2.0-85070705746
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/766/1801.pdf
dc.identifier.rcubconv_2513
dc.type.versionpublishedVersion


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