Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle
Само за регистроване кориснике
2020
Аутори
Stojić, VanjaGlisić, Branislav
Đukić, Ljiljana
Prokić, Bogomir Bolka
Janović, Aleksa
Stamenković, Zorana
Milutinović-Smiljanić, Sanja
Danilović, Vesna
Brković, Božidar
Roganović, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities ar...ea were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.
Кључне речи:
Lateral mandibular deviation / VEGF / Oxidative stress / nNOS / m. Masseter / Synovial membrane / CondyleИзвор:
Archives of Oral Biology, 2020, 110, 104599-Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
- Контрола бола и молекуларни механизми као фактори регенеративне терапије у стоматологији код здравих и пацијената са дијабетес мелитусом (RS-MESTD-Basic Research (BR or ON)-175021)
DOI: 10.1016/j.archoralbio.2019.104599
ISSN: 0003-9969
PubMed: 31734543
WoS: 000523654000017
Scopus: 2-s2.0-85074883629
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Stojić, Vanja AU - Glisić, Branislav AU - Đukić, Ljiljana AU - Prokić, Bogomir Bolka AU - Janović, Aleksa AU - Stamenković, Zorana AU - Milutinović-Smiljanić, Sanja AU - Danilović, Vesna AU - Brković, Božidar AU - Roganović, Jelena PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1820 AB - Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Archives of Oral Biology T1 - Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle VL - 110 SP - 104599 DO - 10.1016/j.archoralbio.2019.104599 ER -
@article{ author = "Stojić, Vanja and Glisić, Branislav and Đukić, Ljiljana and Prokić, Bogomir Bolka and Janović, Aleksa and Stamenković, Zorana and Milutinović-Smiljanić, Sanja and Danilović, Vesna and Brković, Božidar and Roganović, Jelena", year = "2020", abstract = "Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Archives of Oral Biology", title = "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle", volume = "110", pages = "104599", doi = "10.1016/j.archoralbio.2019.104599" }
Stojić, V., Glisić, B., Đukić, L., Prokić, B. B., Janović, A., Stamenković, Z., Milutinović-Smiljanić, S., Danilović, V., Brković, B.,& Roganović, J.. (2020). Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology Pergamon-Elsevier Science Ltd, Oxford., 110, 104599. https://doi.org/10.1016/j.archoralbio.2019.104599
Stojić V, Glisić B, Đukić L, Prokić BB, Janović A, Stamenković Z, Milutinović-Smiljanić S, Danilović V, Brković B, Roganović J. Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology. 2020;110:104599. doi:10.1016/j.archoralbio.2019.104599 .
Stojić, Vanja, Glisić, Branislav, Đukić, Ljiljana, Prokić, Bogomir Bolka, Janović, Aleksa, Stamenković, Zorana, Milutinović-Smiljanić, Sanja, Danilović, Vesna, Brković, Božidar, Roganović, Jelena, "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle" in Archives of Oral Biology, 110 (2020):104599, https://doi.org/10.1016/j.archoralbio.2019.104599 . .