Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals
Autori
Loncarić, IgorKuebber-Heiss, Anna
Posautz, Annika
Ruppitsch, Werner
Lepuschitz, Sarah
Schauer, Bernhard
Fessler, Andrea T.
Krametter-Froetscher, Reinhild
Harrison, Ewan M.
Holmes, Mark A.
Kuenzel, Frank
Szostak, Michael
Hauschild, Tomasz
Desvars-Larrive, Amelie
Mišić, Dušan
Rosengarten, Renate
Walzer, Chris
Slickers, Peter
Monecke, Stefan
Ehricht, Ralf
Schwarz, Stefan
Spergser, Joachim
Članak u časopisu (Recenzirana verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found t...o be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.
Ključne reči:
mecA / mecC / Coagulase-negative Staphylococcus spp. / Animals / Antimicrobial resistanceIzvor:
Veterinary Microbiology, 2019, 230, 138-144Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Research Institute of Wildlife Ecology from University of Veterinary Medicine Vienna, Austria
- Austrian Buiatric association
- Federal Ministry of Education and Research (BMBF) as part of the Research Network Zoonotic Infectious DiseasesFederal Ministry of Education & Research (BMBF) [01KI1727D]
Napomena:
- This is the peer-reviewed version of the article: Loncarić, I.; Kuebber-Heiss, A.; Posautz, A.; Ruppitsch, W.; Lepuschitz, S.; Schauer, B.; Fessler, A. T.; Krametter-Froetscher, R.; Harrison, E. M.; Holmes, M. A.; Kuenzel, F.; Szostak, M.; Hauschild, T.; Desvars-Larrive, A.; Mišić, D.; Rosengarten, R.; Walzer, C.; Slickers, P.; Monecke, S.; Ehricht, R.; Schwarz, S.; Spergser, J. Characterization of MecC Gene-Carrying Coagulase-Negative Staphylococcus Spp. Isolated from Various Animals. Veterinary Microbiology 2019, 230, 138–144. https://doi.org/10.1016/j.vetmic.2019.02.014
DOI: 10.1016/j.vetmic.2019.02.014
ISSN: 0378-1135
PubMed: 30827379
WoS: 000461534600020
Scopus: 2-s2.0-85061198671
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Loncarić, Igor AU - Kuebber-Heiss, Anna AU - Posautz, Annika AU - Ruppitsch, Werner AU - Lepuschitz, Sarah AU - Schauer, Bernhard AU - Fessler, Andrea T. AU - Krametter-Froetscher, Reinhild AU - Harrison, Ewan M. AU - Holmes, Mark A. AU - Kuenzel, Frank AU - Szostak, Michael AU - Hauschild, Tomasz AU - Desvars-Larrive, Amelie AU - Mišić, Dušan AU - Rosengarten, Renate AU - Walzer, Chris AU - Slickers, Peter AU - Monecke, Stefan AU - Ehricht, Ralf AU - Schwarz, Stefan AU - Spergser, Joachim PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1845 AB - The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described. PB - Elsevier, Amsterdam T2 - Veterinary Microbiology T1 - Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals VL - 230 SP - 138 EP - 144 DO - 10.1016/j.vetmic.2019.02.014 ER -
@article{ author = "Loncarić, Igor and Kuebber-Heiss, Anna and Posautz, Annika and Ruppitsch, Werner and Lepuschitz, Sarah and Schauer, Bernhard and Fessler, Andrea T. and Krametter-Froetscher, Reinhild and Harrison, Ewan M. and Holmes, Mark A. and Kuenzel, Frank and Szostak, Michael and Hauschild, Tomasz and Desvars-Larrive, Amelie and Mišić, Dušan and Rosengarten, Renate and Walzer, Chris and Slickers, Peter and Monecke, Stefan and Ehricht, Ralf and Schwarz, Stefan and Spergser, Joachim", year = "2019", abstract = "The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.", publisher = "Elsevier, Amsterdam", journal = "Veterinary Microbiology", title = "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals", volume = "230", pages = "138-144", doi = "10.1016/j.vetmic.2019.02.014" }
Loncarić, I., Kuebber-Heiss, A., Posautz, A., Ruppitsch, W., Lepuschitz, S., Schauer, B., Fessler, A. T., Krametter-Froetscher, R., Harrison, E. M., Holmes, M. A., Kuenzel, F., Szostak, M., Hauschild, T., Desvars-Larrive, A., Mišić, D., Rosengarten, R., Walzer, C., Slickers, P., Monecke, S., Ehricht, R., Schwarz, S.,& Spergser, J.. (2019). Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology Elsevier, Amsterdam., 230, 138-144. https://doi.org/10.1016/j.vetmic.2019.02.014
Loncarić I, Kuebber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Froetscher R, Harrison EM, Holmes MA, Kuenzel F, Szostak M, Hauschild T, Desvars-Larrive A, Mišić D, Rosengarten R, Walzer C, Slickers P, Monecke S, Ehricht R, Schwarz S, Spergser J. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology. 2019;230:138-144. doi:10.1016/j.vetmic.2019.02.014 .
Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals" in Veterinary Microbiology, 230 (2019):138-144, https://doi.org/10.1016/j.vetmic.2019.02.014 . .