Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes
Само за регистроване кориснике
2020
Аутори
Kovačić, MarijanaFratrić, Natalija
Arsić, Aleksandra
Mojsilović, Slavko
Drvenica, Ivana
Marković, Dragana
Maslovarić, Irina
Grujić Milanović, Jelica
Ivanov, Milan
Ilić, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering disp...layed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.
Кључне речи:
Calves bronchopneumonia / IgG / Immune complexes / Leukocytes / LipidsИзвор:
Research in Veterinary Science, 2020, 133, 63-74Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200015 (Универзитет у Београду, Институт за медицинска истраживања) (RS-MESTD-inst-2020-200015)
DOI: 10.1016/j.rvsc.2020.09.004
ISSN: 0034-5288
WoS: 000600569200012
Scopus: 2-s2.0-85090714328
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kovačić, Marijana AU - Fratrić, Natalija AU - Arsić, Aleksandra AU - Mojsilović, Slavko AU - Drvenica, Ivana AU - Marković, Dragana AU - Maslovarić, Irina AU - Grujić Milanović, Jelica AU - Ivanov, Milan AU - Ilić, Vesna PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1862 AB - Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function. PB - Elsevier T2 - Research in Veterinary Science T1 - Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes VL - 133 SP - 63 EP - 74 DO - 10.1016/j.rvsc.2020.09.004 ER -
@article{ author = "Kovačić, Marijana and Fratrić, Natalija and Arsić, Aleksandra and Mojsilović, Slavko and Drvenica, Ivana and Marković, Dragana and Maslovarić, Irina and Grujić Milanović, Jelica and Ivanov, Milan and Ilić, Vesna", year = "2020", abstract = "Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.", publisher = "Elsevier", journal = "Research in Veterinary Science", title = "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes", volume = "133", pages = "63-74", doi = "10.1016/j.rvsc.2020.09.004" }
Kovačić, M., Fratrić, N., Arsić, A., Mojsilović, S., Drvenica, I., Marković, D., Maslovarić, I., Grujić Milanović, J., Ivanov, M.,& Ilić, V.. (2020). Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science Elsevier., 133, 63-74. https://doi.org/10.1016/j.rvsc.2020.09.004
Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić Milanović J, Ivanov M, Ilić V. Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science. 2020;133:63-74. doi:10.1016/j.rvsc.2020.09.004 .
Kovačić, Marijana, Fratrić, Natalija, Arsić, Aleksandra, Mojsilović, Slavko, Drvenica, Ivana, Marković, Dragana, Maslovarić, Irina, Grujić Milanović, Jelica, Ivanov, Milan, Ilić, Vesna, "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes" in Research in Veterinary Science, 133 (2020):63-74, https://doi.org/10.1016/j.rvsc.2020.09.004 . .