Comparative analysis of antibiotic residue in milk using enzyme and microbiological methods
Uporedno ispitivanje rezidua antibiotika u mleku enzimskom i mikrobiološkim metodama
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Antibiotic residue can have a harmful effect on human health and can disrupt the processing of milk and milk products. In order to prevent these unwanted effects of residue, different screening methods are used today. The basic goal of this paper is to compare screening methods performed during the testing of milk from different points of the production chain. In this paper we have comparatively analyzed three screening methods: microbiological methods - the Delvo SP test and the diffusion method with B. stearothermophilus as the test microorganism, and an enzyme method - the Penzym S test. Twenty samples of farm milk from collective tanks were analyzed, as well as 20 samples of milk from transport cisterns, 10 samples of pasteurized milk and 10 samples of sterilized market milk. Based on the comparative analysis of the diffusion method, Delvo SP test and Panzym S test, we conclude that all three methods are in hgih mutual accordance (the kappa value oscillates from nearly ideal to ide...al coinciding) and thus meet one of the criteria for being included in the systematic control of milk for the presence of antibiotic residue.
Rezidue antibiotika mogu štetno da deluju i na zdravlje ljudi i ometaju preradu mleka u proizvode. Da bi se sprečili ovi nepoželjni efekti rezidua, danas se koriste različite skrining metode. Osnovni zadatak ovoga rada je poređenje skrining metoda prilikom ispitivanja mleka sa različitih mesta u lancu proizvodnje. U radu smo uporedo ispitali tri skrining metode: mikrobiološke metode Delvo SP test i Difuzionu metodu sa B. stearothermophilus kao test mikroorganizmom i enzimsku metodu Penzym S test. Ispitano je 20 uzoraka mleka iz sabirnih tankova sa farme, 20 uzoraka mleka iz transportnih cisterni, 10 uzoraka pasterizovanog mleka i 10 uzoraka sterilizovanog mleka iz prometa. Na osnovu uporednih ispitivanja Difuzione metode, Delvo SP testa i Penzym S testa zaključujemo da su sve tri metode u visokoj međusobnoj saglasnosti (kappa vrednost se kreće od skoro idealne podudarnosti do idealne podudarnosti) i time ispunjavaju jedan od kriterijuma za uključivanje u sistematsku kontrolu mleka na p...risustvo rezidua antibiotika.
Кључне речи:
milk / residue / antibiotics / diffusion method / Delvo SP test / Penzym S test / mleko / rezidue / antibiotici / Difuziona metodaИзвор:
Veterinarski Glasnik, 2003, 57, 1-2, 43-49Издавач:
- Fakultet veterinarske medicine, Beograd, Srbija
URI
https://doaj.org/article/663fd2adde9b42f5823fa8fc3f99c40fhttps://vet-erinar.vet.bg.ac.rs/handle/123456789/1893
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Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Petrović, Jelena AU - Katić, Vera R. PY - 2003 UR - https://doaj.org/article/663fd2adde9b42f5823fa8fc3f99c40f UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1893 AB - Antibiotic residue can have a harmful effect on human health and can disrupt the processing of milk and milk products. In order to prevent these unwanted effects of residue, different screening methods are used today. The basic goal of this paper is to compare screening methods performed during the testing of milk from different points of the production chain. In this paper we have comparatively analyzed three screening methods: microbiological methods - the Delvo SP test and the diffusion method with B. stearothermophilus as the test microorganism, and an enzyme method - the Penzym S test. Twenty samples of farm milk from collective tanks were analyzed, as well as 20 samples of milk from transport cisterns, 10 samples of pasteurized milk and 10 samples of sterilized market milk. Based on the comparative analysis of the diffusion method, Delvo SP test and Panzym S test, we conclude that all three methods are in hgih mutual accordance (the kappa value oscillates from nearly ideal to ideal coinciding) and thus meet one of the criteria for being included in the systematic control of milk for the presence of antibiotic residue. AB - Rezidue antibiotika mogu štetno da deluju i na zdravlje ljudi i ometaju preradu mleka u proizvode. Da bi se sprečili ovi nepoželjni efekti rezidua, danas se koriste različite skrining metode. Osnovni zadatak ovoga rada je poređenje skrining metoda prilikom ispitivanja mleka sa različitih mesta u lancu proizvodnje. U radu smo uporedo ispitali tri skrining metode: mikrobiološke metode Delvo SP test i Difuzionu metodu sa B. stearothermophilus kao test mikroorganizmom i enzimsku metodu Penzym S test. Ispitano je 20 uzoraka mleka iz sabirnih tankova sa farme, 20 uzoraka mleka iz transportnih cisterni, 10 uzoraka pasterizovanog mleka i 10 uzoraka sterilizovanog mleka iz prometa. Na osnovu uporednih ispitivanja Difuzione metode, Delvo SP testa i Penzym S testa zaključujemo da su sve tri metode u visokoj međusobnoj saglasnosti (kappa vrednost se kreće od skoro idealne podudarnosti do idealne podudarnosti) i time ispunjavaju jedan od kriterijuma za uključivanje u sistematsku kontrolu mleka na prisustvo rezidua antibiotika. PB - Fakultet veterinarske medicine, Beograd, Srbija T2 - Veterinarski Glasnik T1 - Comparative analysis of antibiotic residue in milk using enzyme and microbiological methods VL - 57 IS - 1-2 SP - 43 EP - 49 DO - 10.2298/VETGL0302043P ER -
@article{ author = "Petrović, Jelena and Katić, Vera R.", year = "2003", abstract = "Antibiotic residue can have a harmful effect on human health and can disrupt the processing of milk and milk products. In order to prevent these unwanted effects of residue, different screening methods are used today. The basic goal of this paper is to compare screening methods performed during the testing of milk from different points of the production chain. In this paper we have comparatively analyzed three screening methods: microbiological methods - the Delvo SP test and the diffusion method with B. stearothermophilus as the test microorganism, and an enzyme method - the Penzym S test. Twenty samples of farm milk from collective tanks were analyzed, as well as 20 samples of milk from transport cisterns, 10 samples of pasteurized milk and 10 samples of sterilized market milk. Based on the comparative analysis of the diffusion method, Delvo SP test and Panzym S test, we conclude that all three methods are in hgih mutual accordance (the kappa value oscillates from nearly ideal to ideal coinciding) and thus meet one of the criteria for being included in the systematic control of milk for the presence of antibiotic residue., Rezidue antibiotika mogu štetno da deluju i na zdravlje ljudi i ometaju preradu mleka u proizvode. Da bi se sprečili ovi nepoželjni efekti rezidua, danas se koriste različite skrining metode. Osnovni zadatak ovoga rada je poređenje skrining metoda prilikom ispitivanja mleka sa različitih mesta u lancu proizvodnje. U radu smo uporedo ispitali tri skrining metode: mikrobiološke metode Delvo SP test i Difuzionu metodu sa B. stearothermophilus kao test mikroorganizmom i enzimsku metodu Penzym S test. Ispitano je 20 uzoraka mleka iz sabirnih tankova sa farme, 20 uzoraka mleka iz transportnih cisterni, 10 uzoraka pasterizovanog mleka i 10 uzoraka sterilizovanog mleka iz prometa. Na osnovu uporednih ispitivanja Difuzione metode, Delvo SP testa i Penzym S testa zaključujemo da su sve tri metode u visokoj međusobnoj saglasnosti (kappa vrednost se kreće od skoro idealne podudarnosti do idealne podudarnosti) i time ispunjavaju jedan od kriterijuma za uključivanje u sistematsku kontrolu mleka na prisustvo rezidua antibiotika.", publisher = "Fakultet veterinarske medicine, Beograd, Srbija", journal = "Veterinarski Glasnik", title = "Comparative analysis of antibiotic residue in milk using enzyme and microbiological methods", volume = "57", number = "1-2", pages = "43-49", doi = "10.2298/VETGL0302043P" }
Petrović, J.,& Katić, V. R.. (2003). Comparative analysis of antibiotic residue in milk using enzyme and microbiological methods. in Veterinarski Glasnik Fakultet veterinarske medicine, Beograd, Srbija., 57(1-2), 43-49. https://doi.org/10.2298/VETGL0302043P
Petrović J, Katić VR. Comparative analysis of antibiotic residue in milk using enzyme and microbiological methods. in Veterinarski Glasnik. 2003;57(1-2):43-49. doi:10.2298/VETGL0302043P .
Petrović, Jelena, Katić, Vera R., "Comparative analysis of antibiotic residue in milk using enzyme and microbiological methods" in Veterinarski Glasnik, 57, no. 1-2 (2003):43-49, https://doi.org/10.2298/VETGL0302043P . .