Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs

Abstract

Cilj ovog rada je bilo ispitivanje prisustva i zastupljenosti streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od zdravih, zaklanih i uginulih svinja različitih kategorija sa nekoliko farmi i klanica u Republici Srbiji. Ukupno je ispitano 226 uzoraka briseva tonzila i nosa od klinički zdrave prasadi u tovu, briseva tonzila prasadi starosti 5-20 dana, delova nazofaringealnih tonzila tovljenika zaklanih na klanici, delova nazofaringealnih tonzila prasadi zaklanih na klanici, briseva sa trupova zaklanih svinja na klanici, briseva sa no`eva za evisceraciju organa na klanici, kao i briseva pluća, trbušne duplje i organa naglo uginule prasadi. Ispitivanje prisustva navedenih vrsta bakterija vršeno je primenom standardnih mikrobioloških metoda. Za identifikaciju izolovanih vrsta bakterija korišćeni su komercijalni biohemijski testovi, a za utvrđivanje serotipske pripadnosti izolovanih sojeva Streptococcus suis specifični serumi za kapsularne antigene. Ustanovljeno je da najveći broj izolovanih sojeva pripada rodu Streptococcus, a u daleko manjem broju izolovane bakterije su pripadale rodovima: Enterococcus (6) (10,4%), Aerococcus (3) (6,2%), Lactococcus (2) (4,2%) i Globicatella (2) (4,2%). Od navede nog broja Streptococcus vrsta najveći broj je pripadao vrsti Streptococcus suis (23) (64%). U brisevima nazofaringealnih tonzila utvrđeno je prisustvo i ostalih á hemolitičkih streptokoka: Streptococcus sanguinis (13,8%), Streptococcus salivarius (5,6%), Streptococcus mitis (5,6%), Streptococcus parasanguinis (2,7%) i Streptococcus oralis (2,7%). Takođe, u manjem procentu izolovana je i vrsta Streptococcus bovis (5,6%). Najveći procenat izolovanih bakterija iz roda Enterococcus pripadao je vrsti Enterococcus faecalis (80%), dok je vrsti Enterococcus faecium pripadao znatno manji procenat izolata (20%). Od ostalih takozvanih streptokokama sličnih vrsta bakterija izolovane su: Aerococcus viridans, Globicatella sanguinis i Lactococcus lactis ssp. cremoris.

The aim of this investigation was to establish the presence and prevalence of streptococci, enterococci and streptococci-like bacteria in various materials originating from healthy, slaughtered and dead pigs belonging to different categories from several farms and slaughterhouses in the Republic of Serbia. The total number of investigated samples comprised 226 swabs of tonsils and noses from clinically healthy breeders, swabs of tonsils from piglets 5-20 days old, parts of nasopharyngeal tonsils from breeders slaughtered in a slaughterhouse, parts of nasopharyngeal tonsils from piglets slaughtered in a slaughterhouse, swabs of slaughtered pig carcasses from a slaughterhouse, swabs from knives for evisceration in a slaughterhouse, as well as swabs of lungs, abdominal cavity and organs from piglets which died suddenly. The standard microbiological methods were used for investigations of the presence of the listed microorganisms. Commercial biochemical tests were used for the identification of the isolated bacteria and specific sera for capsular antigenes were used for serological determination of the isolated S. suis strains. It was established that the great majority of the isolated strains belonged to the genus Streptococcus (36) (75%), and the minority of the strains belonged to the following genera: Enterococcus (6) (10.4%), Aerococcus (3) (6.2%), Lactococcus (2) (4.2%) and Globicatella (2) (4.2%). The great majority of Streptococcus species belonged to S. suis. The presence of other á haemolytic streptococci was established in the swabs of nasopharyngeal tonsils: Streptococcus sanguinis (13.8%), Streptococcus salivarius (5.6%), Streptococcus mitis (5.6%), Streptococcus parasanguinis (2.7%) and Streptococcus oralis (2.7%). Also, S. bovis was isolated in a smaller percentage (5.6%). The greatest number of isolated bacteria from the genus Enterococcus belonged to Enterococcus faecalis (80%), while the minority of isolated strains belonged to Enterococcus faecium (20%). The following from the streptococci-like bacteria were isolated: Aerococcus viridans, Globicatella sanguinis and Lactococcus lactis ssp.cremoris.