Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples
Само за регистроване кориснике
2020
Аутори
Atanasković, IvaMarjanović, Đorđe
Trailović, Saša
Fira, Đorđe
Stanković, Slaviša
Lozo, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Natural isolates coming from chemically untreated environmental samples can be a source of new nematocidal bacterial strains. Little is known about the abundance of such isolates; the diversity of nematocidal proteins that they produce, or how their nematocidal activity changes in a growth phase-dependent manner. In this study, a collection of 17 Bacillus thuringiensis (Bt) natural isolates was screened for nematocidal activity and the presence of nematocidal genes (cry1, cry5, cry6, cry12, cry13, cry14, and cry21). Most strains were positive for cry1 and at least one other cry gene. Further, nematocidal activity against Caenorhabditis elegans was detected in 82.35% of strains. This percentage is significantly higher than percentages found in previously reported studies on similar strain collections. We next sought to investigate the correlation between bacterial growth phase and nematocidal activity. All strains clustered into two growth phase-dependent nematocidal activity profiles. ...28.57% of nematocidal strains were active from the exponential and throughout the stationary phase. 42.85% of them were active only in the exponential phase. Strains active only in the stationary phase were not observed. A combination of strains active in different growth phases was then used to investigate the possibility of killing entire nematode populations. Complete clearance of nematodes was accomplished after two days of incubation when a mixed culture of two such strains (SS-9.4 and SS-16.2) was used. Therefore, combining Bt strains, which are nematocidal in different growth phases, could ameliorate their application in the biological control of nematodes.
Кључне речи:
Bacillus thuringiensis / biological control / Caenorhabditis elegans / cry proteins / nematocidal activityИзвор:
Biocontrol Science and Technology, 2020, 30, 11, 1199-1211Издавач:
- Taylor and Francis Ltd.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
DOI: 10.1080/09583157.2020.1802698
ISSN: 0958-3157
WoS: 000555137400001
Scopus: 2-s2.0-85088979839
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Atanasković, Iva AU - Marjanović, Đorđe AU - Trailović, Saša AU - Fira, Đorđe AU - Stanković, Slaviša AU - Lozo, Jelena PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1973 AB - Natural isolates coming from chemically untreated environmental samples can be a source of new nematocidal bacterial strains. Little is known about the abundance of such isolates; the diversity of nematocidal proteins that they produce, or how their nematocidal activity changes in a growth phase-dependent manner. In this study, a collection of 17 Bacillus thuringiensis (Bt) natural isolates was screened for nematocidal activity and the presence of nematocidal genes (cry1, cry5, cry6, cry12, cry13, cry14, and cry21). Most strains were positive for cry1 and at least one other cry gene. Further, nematocidal activity against Caenorhabditis elegans was detected in 82.35% of strains. This percentage is significantly higher than percentages found in previously reported studies on similar strain collections. We next sought to investigate the correlation between bacterial growth phase and nematocidal activity. All strains clustered into two growth phase-dependent nematocidal activity profiles. 28.57% of nematocidal strains were active from the exponential and throughout the stationary phase. 42.85% of them were active only in the exponential phase. Strains active only in the stationary phase were not observed. A combination of strains active in different growth phases was then used to investigate the possibility of killing entire nematode populations. Complete clearance of nematodes was accomplished after two days of incubation when a mixed culture of two such strains (SS-9.4 and SS-16.2) was used. Therefore, combining Bt strains, which are nematocidal in different growth phases, could ameliorate their application in the biological control of nematodes. PB - Taylor and Francis Ltd. T2 - Biocontrol Science and Technology T1 - Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples VL - 30 IS - 11 SP - 1199 EP - 1211 DO - 10.1080/09583157.2020.1802698 ER -
@article{ author = "Atanasković, Iva and Marjanović, Đorđe and Trailović, Saša and Fira, Đorđe and Stanković, Slaviša and Lozo, Jelena", year = "2020", abstract = "Natural isolates coming from chemically untreated environmental samples can be a source of new nematocidal bacterial strains. Little is known about the abundance of such isolates; the diversity of nematocidal proteins that they produce, or how their nematocidal activity changes in a growth phase-dependent manner. In this study, a collection of 17 Bacillus thuringiensis (Bt) natural isolates was screened for nematocidal activity and the presence of nematocidal genes (cry1, cry5, cry6, cry12, cry13, cry14, and cry21). Most strains were positive for cry1 and at least one other cry gene. Further, nematocidal activity against Caenorhabditis elegans was detected in 82.35% of strains. This percentage is significantly higher than percentages found in previously reported studies on similar strain collections. We next sought to investigate the correlation between bacterial growth phase and nematocidal activity. All strains clustered into two growth phase-dependent nematocidal activity profiles. 28.57% of nematocidal strains were active from the exponential and throughout the stationary phase. 42.85% of them were active only in the exponential phase. Strains active only in the stationary phase were not observed. A combination of strains active in different growth phases was then used to investigate the possibility of killing entire nematode populations. Complete clearance of nematodes was accomplished after two days of incubation when a mixed culture of two such strains (SS-9.4 and SS-16.2) was used. Therefore, combining Bt strains, which are nematocidal in different growth phases, could ameliorate their application in the biological control of nematodes.", publisher = "Taylor and Francis Ltd.", journal = "Biocontrol Science and Technology", title = "Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples", volume = "30", number = "11", pages = "1199-1211", doi = "10.1080/09583157.2020.1802698" }
Atanasković, I., Marjanović, Đ., Trailović, S., Fira, Đ., Stanković, S.,& Lozo, J.. (2020). Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples. in Biocontrol Science and Technology Taylor and Francis Ltd.., 30(11), 1199-1211. https://doi.org/10.1080/09583157.2020.1802698
Atanasković I, Marjanović Đ, Trailović S, Fira Đ, Stanković S, Lozo J. Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples. in Biocontrol Science and Technology. 2020;30(11):1199-1211. doi:10.1080/09583157.2020.1802698 .
Atanasković, Iva, Marjanović, Đorđe, Trailović, Saša, Fira, Đorđe, Stanković, Slaviša, Lozo, Jelena, "Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples" in Biocontrol Science and Technology, 30, no. 11 (2020):1199-1211, https://doi.org/10.1080/09583157.2020.1802698 . .