External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions
2020
Аутори
Pérez-Ramírez, ElisaCano-Gómez, Cristina
Llorente, Francisco
Adžić, Bojan
Al Ameer, Maisa
Djadjovski, Igor
Hage, Jeanne El
Mellouli, Fatiha El
Goletic, Teufik
Hovsepyan, Hermine
Karayel-Hacioglu, Ilke
Maksimović-Zorić, Jelena
Mejri, Selma
Sadaoui, Hassiba
Salem, Sayed Hassan
Sherifi, Kurtesh
Toklikishvili, Natela
Vodica, Ani
Monaco, Federica
Brun, Alejandro
Jiménez-Clavero, Miguel Ángel
Fernández-Pinero, Jovita
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventee...n veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.
Извор:
PLoS ONE, 2020, 15Издавач:
- Public Library of Science
DOI: 10.1371/journal.pone.0239478
ISSN: 1932-6203
WoS: 000576267900073
Scopus: 2-s2.0-85092056278
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Pérez-Ramírez, Elisa AU - Cano-Gómez, Cristina AU - Llorente, Francisco AU - Adžić, Bojan AU - Al Ameer, Maisa AU - Djadjovski, Igor AU - Hage, Jeanne El AU - Mellouli, Fatiha El AU - Goletic, Teufik AU - Hovsepyan, Hermine AU - Karayel-Hacioglu, Ilke AU - Maksimović-Zorić, Jelena AU - Mejri, Selma AU - Sadaoui, Hassiba AU - Salem, Sayed Hassan AU - Sherifi, Kurtesh AU - Toklikishvili, Natela AU - Vodica, Ani AU - Monaco, Federica AU - Brun, Alejandro AU - Jiménez-Clavero, Miguel Ángel AU - Fernández-Pinero, Jovita PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1986 AB - Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples. PB - Public Library of Science T2 - PLoS ONE T1 - External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions VL - 15 DO - 10.1371/journal.pone.0239478 ER -
@article{ author = "Pérez-Ramírez, Elisa and Cano-Gómez, Cristina and Llorente, Francisco and Adžić, Bojan and Al Ameer, Maisa and Djadjovski, Igor and Hage, Jeanne El and Mellouli, Fatiha El and Goletic, Teufik and Hovsepyan, Hermine and Karayel-Hacioglu, Ilke and Maksimović-Zorić, Jelena and Mejri, Selma and Sadaoui, Hassiba and Salem, Sayed Hassan and Sherifi, Kurtesh and Toklikishvili, Natela and Vodica, Ani and Monaco, Federica and Brun, Alejandro and Jiménez-Clavero, Miguel Ángel and Fernández-Pinero, Jovita", year = "2020", abstract = "Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.", publisher = "Public Library of Science", journal = "PLoS ONE", title = "External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions", volume = "15", doi = "10.1371/journal.pone.0239478" }
Pérez-Ramírez, E., Cano-Gómez, C., Llorente, F., Adžić, B., Al Ameer, M., Djadjovski, I., Hage, J. E., Mellouli, F. E., Goletic, T., Hovsepyan, H., Karayel-Hacioglu, I., Maksimović-Zorić, J., Mejri, S., Sadaoui, H., Salem, S. H., Sherifi, K., Toklikishvili, N., Vodica, A., Monaco, F., Brun, A., Jiménez-Clavero, M. Á.,& Fernández-Pinero, J.. (2020). External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions. in PLoS ONE Public Library of Science., 15. https://doi.org/10.1371/journal.pone.0239478
Pérez-Ramírez E, Cano-Gómez C, Llorente F, Adžić B, Al Ameer M, Djadjovski I, Hage JE, Mellouli FE, Goletic T, Hovsepyan H, Karayel-Hacioglu I, Maksimović-Zorić J, Mejri S, Sadaoui H, Salem SH, Sherifi K, Toklikishvili N, Vodica A, Monaco F, Brun A, Jiménez-Clavero MÁ, Fernández-Pinero J. External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions. in PLoS ONE. 2020;15. doi:10.1371/journal.pone.0239478 .
Pérez-Ramírez, Elisa, Cano-Gómez, Cristina, Llorente, Francisco, Adžić, Bojan, Al Ameer, Maisa, Djadjovski, Igor, Hage, Jeanne El, Mellouli, Fatiha El, Goletic, Teufik, Hovsepyan, Hermine, Karayel-Hacioglu, Ilke, Maksimović-Zorić, Jelena, Mejri, Selma, Sadaoui, Hassiba, Salem, Sayed Hassan, Sherifi, Kurtesh, Toklikishvili, Natela, Vodica, Ani, Monaco, Federica, Brun, Alejandro, Jiménez-Clavero, Miguel Ángel, Fernández-Pinero, Jovita, "External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions" in PLoS ONE, 15 (2020), https://doi.org/10.1371/journal.pone.0239478 . .