Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions
2020
Аутори
Pérez-Ramírez, ElisaCano-Gómez, Cristina
Llorente, Francisco
Vodica, Ani
Veljović, Ljubiša
Toklikishvilli, Natela
Sherifi, Kurtesh
Sghaier, Soufien
Omani, Amel
Kustura, Aida
Krstevski, Kiril
Karayel-Hacioglu, Ilke
Hagag, Naglaa Mohamed
Hage, Jeanne El
Davdyan, Hasmik
Bintarif, Mohd Saddam
Adžić, Bojan
Abouchoaib, Nabil
Jiménez-Clavero, Miguel Ángel
Fernández-Pinero, Jovita
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses usin...g conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.
Кључне речи:
Diagnostics / ELISA / External quality assessment (EQA) / Flavivirus / MediLabSecure / PCR / Usutu virus (USUV) / West Nile virus (WNV)Извор:
Pathogens, 2020, 9, 12, 1-20Издавач:
- MDPI AG
DOI: 10.3390/pathogens9121038
ISSN: 2076-0817
WoS: 000602425500001
Scopus: 2-s2.0-85097681600
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Pérez-Ramírez, Elisa AU - Cano-Gómez, Cristina AU - Llorente, Francisco AU - Vodica, Ani AU - Veljović, Ljubiša AU - Toklikishvilli, Natela AU - Sherifi, Kurtesh AU - Sghaier, Soufien AU - Omani, Amel AU - Kustura, Aida AU - Krstevski, Kiril AU - Karayel-Hacioglu, Ilke AU - Hagag, Naglaa Mohamed AU - Hage, Jeanne El AU - Davdyan, Hasmik AU - Bintarif, Mohd Saddam AU - Adžić, Bojan AU - Abouchoaib, Nabil AU - Jiménez-Clavero, Miguel Ángel AU - Fernández-Pinero, Jovita PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2019 AB - The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities. PB - MDPI AG T2 - Pathogens T1 - Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions VL - 9 IS - 12 SP - 1 EP - 20 DO - 10.3390/pathogens9121038 ER -
@article{ author = "Pérez-Ramírez, Elisa and Cano-Gómez, Cristina and Llorente, Francisco and Vodica, Ani and Veljović, Ljubiša and Toklikishvilli, Natela and Sherifi, Kurtesh and Sghaier, Soufien and Omani, Amel and Kustura, Aida and Krstevski, Kiril and Karayel-Hacioglu, Ilke and Hagag, Naglaa Mohamed and Hage, Jeanne El and Davdyan, Hasmik and Bintarif, Mohd Saddam and Adžić, Bojan and Abouchoaib, Nabil and Jiménez-Clavero, Miguel Ángel and Fernández-Pinero, Jovita", year = "2020", abstract = "The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.", publisher = "MDPI AG", journal = "Pathogens", title = "Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions", volume = "9", number = "12", pages = "1-20", doi = "10.3390/pathogens9121038" }
Pérez-Ramírez, E., Cano-Gómez, C., Llorente, F., Vodica, A., Veljović, L., Toklikishvilli, N., Sherifi, K., Sghaier, S., Omani, A., Kustura, A., Krstevski, K., Karayel-Hacioglu, I., Hagag, N. M., Hage, J. E., Davdyan, H., Bintarif, M. S., Adžić, B., Abouchoaib, N., Jiménez-Clavero, M. Á.,& Fernández-Pinero, J.. (2020). Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions. in Pathogens MDPI AG., 9(12), 1-20. https://doi.org/10.3390/pathogens9121038
Pérez-Ramírez E, Cano-Gómez C, Llorente F, Vodica A, Veljović L, Toklikishvilli N, Sherifi K, Sghaier S, Omani A, Kustura A, Krstevski K, Karayel-Hacioglu I, Hagag NM, Hage JE, Davdyan H, Bintarif MS, Adžić B, Abouchoaib N, Jiménez-Clavero MÁ, Fernández-Pinero J. Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions. in Pathogens. 2020;9(12):1-20. doi:10.3390/pathogens9121038 .
Pérez-Ramírez, Elisa, Cano-Gómez, Cristina, Llorente, Francisco, Vodica, Ani, Veljović, Ljubiša, Toklikishvilli, Natela, Sherifi, Kurtesh, Sghaier, Soufien, Omani, Amel, Kustura, Aida, Krstevski, Kiril, Karayel-Hacioglu, Ilke, Hagag, Naglaa Mohamed, Hage, Jeanne El, Davdyan, Hasmik, Bintarif, Mohd Saddam, Adžić, Bojan, Abouchoaib, Nabil, Jiménez-Clavero, Miguel Ángel, Fernández-Pinero, Jovita, "Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions" in Pathogens, 9, no. 12 (2020):1-20, https://doi.org/10.3390/pathogens9121038 . .