Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog
Upotreba mikrosatelita u proceni genetičkog diverziteta, kontroli roditeljstva i individualnoj identifikaciji pasarase kangal
2020
Аутори
Dimitrijević, VladimirRistanić, Marko
Stanišić, Ljubodrag
Drobnjak, Darko
Urošević, Milivoje
Ozkanal, Umit
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin.
This study investigated variations in ten autosomal microsatellite markers (PEZ01,
PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079)
for the purposes of genetic diversity assessment of the Kangal breed. In addition, the
use of markers was assessed in parentage testing and individual identification within
the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total
number of alleles in the study population was 69. The mean number of alleles per locus
was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information
content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value
for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied
between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the
efficiency of using microsatellites for individual identification in the Kang...al breed,
power of discrimination (PD) and matching probability (MP) were calculated for each
microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high
combined PD value of 99.99999%. The obtained results may contribute to further
recognition of the Kangal breed, and confirm that the investigated microsatellites
enable a reliable parentage testing and individual identification of the breed.
Kangal rasa pasa se smatra jednom od najčešćih rasa turskog porekla. Ova studija
je istraživala varijacije deset autozomalnih mikrosatelitskih markera (PEZ01, PEZ03,
PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 i FHC2079) u svrhu
određivanja genetičkog diverziteta pasa rase kangal. Pored toga, izvršena je procena
mogućnosti primene ovih markera u individualnoj identifikaciji i u kontroli roditeljstva pasa rase kangal. Izvršena je tipizacija navedenih mikrosatelitskih markera kod 51
jedinke kangal rase. Ukupan broj alela u ispitivanoj populaciji iznosio je 69. Prosečan
broj alela po lokusu iznosio je 6,9 sa varijacijama od četiri (FHC2079) do 12 (PEZ12).
Polimorfizam (PIC) je varirao od 0,52 (FHC2079) do 0,87 (PEZ12), sa srednjom
vrednošću od 0,717 za sve lokuse. Verovatnoća isključenja (PE) 10 mikrosatelitskih
markera varirala je između 0,143 (FHC2054) i 0,472 (PEZ08) po lokusu. U cilju utvrđivanja efikasnosti u svrhu individualne identifikacije pasa rase kangal, određi...vana je
moć diskriminacije (PD) kao i verovatnoća podudaranja (MP) za svaki od ispitivanih markera. Ispitivani panel postigao je visoke vrednosti kako kombinovane MP (6,77 ×
10-10) tako i kombinovane PD od 99,99999%. Dobijeni rezultati predstavljaju doprinos
genetičkoj karakterizaciji rase kangal i pokazuju da je ispitivani panel mikrosatelitskih
markera pouzdan u kontroli spornih rodbinskih odnosa i u individualnoj identifikaciji
pasa ove rase.
Кључне речи:
allele frequency / canine microsatellites / genetic characterization / Kangal / parentage testingИзвор:
Acta Veterinaria-Beograd, 2020, 70, 2, 170-181Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2478/acve-2020-0012
ISSN: 0567-8315
WoS: 000540849900002
Scopus: 2-s2.0-85087287004
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Dimitrijević, Vladimir AU - Ristanić, Marko AU - Stanišić, Ljubodrag AU - Drobnjak, Darko AU - Urošević, Milivoje AU - Ozkanal, Umit AU - Stanimirović, Zoran PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2039 AB - The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin. This study investigated variations in ten autosomal microsatellite markers (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079) for the purposes of genetic diversity assessment of the Kangal breed. In addition, the use of markers was assessed in parentage testing and individual identification within the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total number of alleles in the study population was 69. The mean number of alleles per locus was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the efficiency of using microsatellites for individual identification in the Kangal breed, power of discrimination (PD) and matching probability (MP) were calculated for each microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high combined PD value of 99.99999%. The obtained results may contribute to further recognition of the Kangal breed, and confirm that the investigated microsatellites enable a reliable parentage testing and individual identification of the breed. AB - Kangal rasa pasa se smatra jednom od najčešćih rasa turskog porekla. Ova studija je istraživala varijacije deset autozomalnih mikrosatelitskih markera (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 i FHC2079) u svrhu određivanja genetičkog diverziteta pasa rase kangal. Pored toga, izvršena je procena mogućnosti primene ovih markera u individualnoj identifikaciji i u kontroli roditeljstva pasa rase kangal. Izvršena je tipizacija navedenih mikrosatelitskih markera kod 51 jedinke kangal rase. Ukupan broj alela u ispitivanoj populaciji iznosio je 69. Prosečan broj alela po lokusu iznosio je 6,9 sa varijacijama od četiri (FHC2079) do 12 (PEZ12). Polimorfizam (PIC) je varirao od 0,52 (FHC2079) do 0,87 (PEZ12), sa srednjom vrednošću od 0,717 za sve lokuse. Verovatnoća isključenja (PE) 10 mikrosatelitskih markera varirala je između 0,143 (FHC2054) i 0,472 (PEZ08) po lokusu. U cilju utvrđivanja efikasnosti u svrhu individualne identifikacije pasa rase kangal, određivana je moć diskriminacije (PD) kao i verovatnoća podudaranja (MP) za svaki od ispitivanih markera. Ispitivani panel postigao je visoke vrednosti kako kombinovane MP (6,77 × 10-10) tako i kombinovane PD od 99,99999%. Dobijeni rezultati predstavljaju doprinos genetičkoj karakterizaciji rase kangal i pokazuju da je ispitivani panel mikrosatelitskih markera pouzdan u kontroli spornih rodbinskih odnosa i u individualnoj identifikaciji pasa ove rase. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog T1 - Upotreba mikrosatelita u proceni genetičkog diverziteta, kontroli roditeljstva i individualnoj identifikaciji pasarase kangal VL - 70 IS - 2 SP - 170 EP - 181 DO - 10.2478/acve-2020-0012 ER -
@article{ author = "Dimitrijević, Vladimir and Ristanić, Marko and Stanišić, Ljubodrag and Drobnjak, Darko and Urošević, Milivoje and Ozkanal, Umit and Stanimirović, Zoran", year = "2020", abstract = "The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin. This study investigated variations in ten autosomal microsatellite markers (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079) for the purposes of genetic diversity assessment of the Kangal breed. In addition, the use of markers was assessed in parentage testing and individual identification within the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total number of alleles in the study population was 69. The mean number of alleles per locus was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the efficiency of using microsatellites for individual identification in the Kangal breed, power of discrimination (PD) and matching probability (MP) were calculated for each microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high combined PD value of 99.99999%. The obtained results may contribute to further recognition of the Kangal breed, and confirm that the investigated microsatellites enable a reliable parentage testing and individual identification of the breed., Kangal rasa pasa se smatra jednom od najčešćih rasa turskog porekla. Ova studija je istraživala varijacije deset autozomalnih mikrosatelitskih markera (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 i FHC2079) u svrhu određivanja genetičkog diverziteta pasa rase kangal. Pored toga, izvršena je procena mogućnosti primene ovih markera u individualnoj identifikaciji i u kontroli roditeljstva pasa rase kangal. Izvršena je tipizacija navedenih mikrosatelitskih markera kod 51 jedinke kangal rase. Ukupan broj alela u ispitivanoj populaciji iznosio je 69. Prosečan broj alela po lokusu iznosio je 6,9 sa varijacijama od četiri (FHC2079) do 12 (PEZ12). Polimorfizam (PIC) je varirao od 0,52 (FHC2079) do 0,87 (PEZ12), sa srednjom vrednošću od 0,717 za sve lokuse. Verovatnoća isključenja (PE) 10 mikrosatelitskih markera varirala je između 0,143 (FHC2054) i 0,472 (PEZ08) po lokusu. U cilju utvrđivanja efikasnosti u svrhu individualne identifikacije pasa rase kangal, određivana je moć diskriminacije (PD) kao i verovatnoća podudaranja (MP) za svaki od ispitivanih markera. Ispitivani panel postigao je visoke vrednosti kako kombinovane MP (6,77 × 10-10) tako i kombinovane PD od 99,99999%. Dobijeni rezultati predstavljaju doprinos genetičkoj karakterizaciji rase kangal i pokazuju da je ispitivani panel mikrosatelitskih markera pouzdan u kontroli spornih rodbinskih odnosa i u individualnoj identifikaciji pasa ove rase.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog, Upotreba mikrosatelita u proceni genetičkog diverziteta, kontroli roditeljstva i individualnoj identifikaciji pasarase kangal", volume = "70", number = "2", pages = "170-181", doi = "10.2478/acve-2020-0012" }
Dimitrijević, V., Ristanić, M., Stanišić, L., Drobnjak, D., Urošević, M., Ozkanal, U.,& Stanimirović, Z.. (2020). Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(2), 170-181. https://doi.org/10.2478/acve-2020-0012
Dimitrijević V, Ristanić M, Stanišić L, Drobnjak D, Urošević M, Ozkanal U, Stanimirović Z. Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog. in Acta Veterinaria-Beograd. 2020;70(2):170-181. doi:10.2478/acve-2020-0012 .
Dimitrijević, Vladimir, Ristanić, Marko, Stanišić, Ljubodrag, Drobnjak, Darko, Urošević, Milivoje, Ozkanal, Umit, Stanimirović, Zoran, "Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog" in Acta Veterinaria-Beograd, 70, no. 2 (2020):170-181, https://doi.org/10.2478/acve-2020-0012 . .