Histological evaluation of periradicular tissue inflammatory reactions and calcified tissue formations after implantation of experimental calcium silicate and hydroxyapatite based nanostructural cements into root canals of rabbits teeth

Abstract

The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured calcium silicate cement (CS) and hydroxyapatite with calcium silicate cement (HA-CS) into root canals of rabbits' teeth. The study was conducted on four rabbits of the genus Oryctolagus cuniculus. After instrumentation and irrigation, the root canals of the central incisors were dried and filled with CS, HA-CS and control material (MTA Angelus). The animals were sacrificed after 28 days. After histological preparation and hematoxylin-eosin staining, tissue samples were evaluated for the intensity and extension of inflammatory tissue reaction; continuity, morphology and thickness of the newly formed calcified tissue; and presence of giant cells, materials particles and microorganisms. Kruskal Wallis and Dunn's post hoc test were used for data analysis (α=0.05). There were no significant differences in the intensity of inflammatory reactions between CS, HA-CS and MTA control. HA-CS showed significantly better results than MTA and CS with respect to continuity of the newly formed calcified tissue (P=0.003 and P=0.010, respectively). Significant differences in thickness of the calcified tissue existed between CS and MTA (P=0.004) and between HA-CS and MTA (P=0.012). Application of CS and HA-CS resulted in minimal inflammatory tissue response, similar to the MTA control. CS and HA-CS were more efficient than MTA in supporting hard tissue formation. The best organized newly formed calcified tissue was seen after HA-CS application.

Cilj rada je bio da se ispitaju Kanali korena centralnih sekutića su posle instrumentacije, ispiranja i sušenja napunjeni materijalima CS, HA-CS i kontrolnim materijalom, mineral trioksid agregatom (MTA). Životinje su žrtvovane posle 28 dana. Posle histološke pripreme, uzorci tkiva su analizirani u pogledu inteziteta i raširenosti zapaljenske reakcije; kontinuiteta, morfologije i debljine novoformiranog kalcifi kovanog tkiva; prisustva džinovskih ćelija, čestica materijala i mikroorganizama. Dobijeni rezultati su statistički obrađeni (α = 0,05) Nisu uočene statistički značajne razlike u intezitetu zapaljenske reakcije između CS, HA-CS i MTA. U pogledu kontinuiteta novostvorenog kalcifi kovanog tkiva HA-CS je pokazao bolje rezultate u odnosu na MTA i CS (p=0,003 i p=0,010). Značajne razlike utvrđene su u pogledu debljine kalcifi kovanog tkiva između CS i MTA (p=0,004), kao i HA-CS i MTA (p=0,012). Aplikacija materijala CS i HA-CS je rezultirala minimalnom zapaljenskom reakcijom tkiva, slično kontrolnom materijalu (MTA). CS i HA-CS su bili efi kasniji u pogledu stimulacije formiranja kalcifi kovanog tkiva u odnosu na MTA. Najbolje organizovano novoformirano tkivo uočeno je posle aplikacije materijala HA-CS. zapaljenske reakcije u periradikularnom tkivu i formiranje kalcifi kovanog tkiva posle implantacije eksperimentalnih, nanostrukturnih cemenata na bazi kalcijum silikata (CS) i mešavine hidroksiapatita i kalcijum silikata (HA-CS) u kanale korena zuba kunića