Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy
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2021
Authors
Došenović, MilanRadaković, Milena
Vučićević, Miloš
Vejnović, Branislav
Vasiljević, Maja
Marinković, Darko
Stanimirović, Zoran
Article (Published version)
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The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine–medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both ana...esthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine–medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol.
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Acta Veterinaria Hungarica, 2021, 68, 4, 337-344Publisher:
- AKJournals
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
DOI: 10.1556/004.2020.00058
ISSN: 1588-2705; 0236-6290
WoS: 000641864600001
Scopus: 2-s2.0-85105633454
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Fakultet veterinarske medicineTY - JOUR AU - Došenović, Milan AU - Radaković, Milena AU - Vučićević, Miloš AU - Vejnović, Branislav AU - Vasiljević, Maja AU - Marinković, Darko AU - Stanimirović, Zoran PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2059 AB - The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine–medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both anaesthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine–medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol. PB - AKJournals T2 - Acta Veterinaria Hungarica T1 - Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy VL - 68 IS - 4 SP - 337 EP - 344 DO - 10.1556/004.2020.00058 ER -
@article{ author = "Došenović, Milan and Radaković, Milena and Vučićević, Miloš and Vejnović, Branislav and Vasiljević, Maja and Marinković, Darko and Stanimirović, Zoran", year = "2021", abstract = "The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine–medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both anaesthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine–medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol.", publisher = "AKJournals", journal = "Acta Veterinaria Hungarica", title = "Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy", volume = "68", number = "4", pages = "337-344", doi = "10.1556/004.2020.00058" }
Došenović, M., Radaković, M., Vučićević, M., Vejnović, B., Vasiljević, M., Marinković, D.,& Stanimirović, Z.. (2021). Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy. in Acta Veterinaria Hungarica AKJournals., 68(4), 337-344. https://doi.org/10.1556/004.2020.00058
Došenović M, Radaković M, Vučićević M, Vejnović B, Vasiljević M, Marinković D, Stanimirović Z. Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy. in Acta Veterinaria Hungarica. 2021;68(4):337-344. doi:10.1556/004.2020.00058 .
Došenović, Milan, Radaković, Milena, Vučićević, Miloš, Vejnović, Branislav, Vasiljević, Maja, Marinković, Darko, Stanimirović, Zoran, "Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy" in Acta Veterinaria Hungarica, 68, no. 4 (2021):337-344, https://doi.org/10.1556/004.2020.00058 . .