Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
Reparacija defekata hrskavice kunića autolognim i alogenim hondrocitima zasejanim na kolagen/ hijaluronan osnovu ili suspendovanim u fibrinskom lepku
Аутори
Horňák, SlavomírHarvanová, Denisa
Ledecký, Valent
Hluchý, Marian
Valenčáková-Agyagosová, Alexandra
Amrichová, Judita
Rosocha, Ján
Vaško, Gabriel
Švihla, Róbert
Petrovič, Vladimír
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The topic of the study was to verify in vivo survival of in vitro cultured autologous and allogenous chondrocytes suspended in a fi brin glue Beriplast® or seeded on Collagen type I-Hyaluronan (Col type I-HYA) scaffolds for the regeneration of articular cartilage defects in rabbits. The study was carried out on 15 domestic rabbits randomly assigned to fi ve groups (n = 3 in each) with different treatments of artifi cially created chondral defects (ChD ́s). These defects were made in a non-load-bearing area of medial condyle of the distal femur, and were treated as follows: 1st and 3rd group: the ChD ́s were fi lled with autologous or allogenous chondrocytes seeded on Col type I-HYA scaffolds, respectively. The scaffolds were fi xed to the ChD ́s by fi brin glue Beriplast®; 2nd and 4th group: the ChD ́s were fi lled with a suspension of autologous or allogenous chondrocytes in fi brin glue ...Beriplast®, respectively, and they were immediately covered by unseeded Col type I-HYA scaffolds; Control group: the ChD ́s were left to heal spontaneously without any treatment. Macroscopical, histological and immunohistochemical analyses of the ChD ́s were performed 12 months after the treatment. In all treated groups, the chondrocytes were capable to proliferate and produce the cartilage extracellular matrix, including proteoglycans and type II collagen, as compared to the control “untreated” group. On the other hand, the production of hyaline-like cartilage tissue confi rmed that both therapeutic methods using autologous chondrocytes can be applied successfully for the treatment of chondral defects in rabbits.
Cilj studije je bio da se verifi kuje in vivo preživljavanje in vitro kultivisanih autolognih i alogenih hondrocita suspendovanih u fi brinski Beriplast® lepak ili na kolagen Hyaluronan tip I (Col type I-HYA) osnovu radi regeneracije oštećenja zglobne hrskavice kod kunića. Ispitivanje je obavljeno na 15 domaćih kunića, odabranih slučajnim izborom koji su podeljeni u pet grupa (3 po grupi) kod kojih su veštački izazvana oštećenja hrskavice (ChD), tretirana na različite načine. Oštećenja su napravljena na površinama koje nisu noseće na medijalnom kondilusu distalnog femura, a tretman je podrazumevao: prva i treća grupa: ChD su punjene sa autolognim ili alogenim hondrocitima zasejanim na Col tip I-HYA osnovu. Osnove su fi ksirane na ChD pomoću fi brinskog lepka Beriplast®; druga i četvrta grupa: oštećenja su punjena suspenzijom autolognih ili alogenih hondrocita u fi brinskom Beriplast® lepku, a neposredno posle toga, prekriveni su sa nezasejanom Col tip I-HYA osnovom; kontrolna grupa: oš...tećenja su ostavljena da spontano zarastu, bez tretmana. Dvanaest meseci posle tretmana obavljene su makroskopske, histološke i imunohistološke analize promena. Kod svih životinja tretiranih grupa, hondrociti su bili sposobni da proliferišu i proizvode ekstracelularni matriks hrskavice, uključujući proteoglikane i II tip kolagena u poređenju sa kontrolnom “netretriranom” grupom. Sa druge strane, proizvodnja hrskavičavog tkiva nalik na hijalin potvrđuje da se oba terapijska metoda koji koriste autologne hondrocite mogu da uspešno primene u tretiranju defekata hrskavice kod kunića.
Кључне речи:
rabbits / articular cartilage defect / chondrocyte implants / collagen / hyaluronanИзвор:
Acta Veterinaria-Beograd, 2014, 64, 1, 61-72Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- This research was supported by VEGA grant no. 1/0406/12 from the Ministry of Education, Science, Research and Sport of the Slovak Republic
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Horňák, Slavomír AU - Harvanová, Denisa AU - Ledecký, Valent AU - Hluchý, Marian AU - Valenčáková-Agyagosová, Alexandra AU - Amrichová, Judita AU - Rosocha, Ján AU - Vaško, Gabriel AU - Švihla, Róbert AU - Petrovič, Vladimír PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2138 AB - The topic of the study was to verify in vivo survival of in vitro cultured autologous and allogenous chondrocytes suspended in a fi brin glue Beriplast® or seeded on Collagen type I-Hyaluronan (Col type I-HYA) scaffolds for the regeneration of articular cartilage defects in rabbits. The study was carried out on 15 domestic rabbits randomly assigned to fi ve groups (n = 3 in each) with different treatments of artifi cially created chondral defects (ChD ́s). These defects were made in a non-load-bearing area of medial condyle of the distal femur, and were treated as follows: 1st and 3rd group: the ChD ́s were fi lled with autologous or allogenous chondrocytes seeded on Col type I-HYA scaffolds, respectively. The scaffolds were fi xed to the ChD ́s by fi brin glue Beriplast®; 2nd and 4th group: the ChD ́s were fi lled with a suspension of autologous or allogenous chondrocytes in fi brin glue Beriplast®, respectively, and they were immediately covered by unseeded Col type I-HYA scaffolds; Control group: the ChD ́s were left to heal spontaneously without any treatment. Macroscopical, histological and immunohistochemical analyses of the ChD ́s were performed 12 months after the treatment. In all treated groups, the chondrocytes were capable to proliferate and produce the cartilage extracellular matrix, including proteoglycans and type II collagen, as compared to the control “untreated” group. On the other hand, the production of hyaline-like cartilage tissue confi rmed that both therapeutic methods using autologous chondrocytes can be applied successfully for the treatment of chondral defects in rabbits. AB - Cilj studije je bio da se verifi kuje in vivo preživljavanje in vitro kultivisanih autolognih i alogenih hondrocita suspendovanih u fi brinski Beriplast® lepak ili na kolagen Hyaluronan tip I (Col type I-HYA) osnovu radi regeneracije oštećenja zglobne hrskavice kod kunića. Ispitivanje je obavljeno na 15 domaćih kunića, odabranih slučajnim izborom koji su podeljeni u pet grupa (3 po grupi) kod kojih su veštački izazvana oštećenja hrskavice (ChD), tretirana na različite načine. Oštećenja su napravljena na površinama koje nisu noseće na medijalnom kondilusu distalnog femura, a tretman je podrazumevao: prva i treća grupa: ChD su punjene sa autolognim ili alogenim hondrocitima zasejanim na Col tip I-HYA osnovu. Osnove su fi ksirane na ChD pomoću fi brinskog lepka Beriplast®; druga i četvrta grupa: oštećenja su punjena suspenzijom autolognih ili alogenih hondrocita u fi brinskom Beriplast® lepku, a neposredno posle toga, prekriveni su sa nezasejanom Col tip I-HYA osnovom; kontrolna grupa: oštećenja su ostavljena da spontano zarastu, bez tretmana. Dvanaest meseci posle tretmana obavljene su makroskopske, histološke i imunohistološke analize promena. Kod svih životinja tretiranih grupa, hondrociti su bili sposobni da proliferišu i proizvode ekstracelularni matriks hrskavice, uključujući proteoglikane i II tip kolagena u poređenju sa kontrolnom “netretriranom” grupom. Sa druge strane, proizvodnja hrskavičavog tkiva nalik na hijalin potvrđuje da se oba terapijska metoda koji koriste autologne hondrocite mogu da uspešno primene u tretiranju defekata hrskavice kod kunića. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue T1 - Reparacija defekata hrskavice kunića autolognim i alogenim hondrocitima zasejanim na kolagen/ hijaluronan osnovu ili suspendovanim u fibrinskom lepku VL - 64 IS - 1 SP - 61 EP - 72 DO - 10.2478/acve-2014-0007 ER -
@article{ author = "Horňák, Slavomír and Harvanová, Denisa and Ledecký, Valent and Hluchý, Marian and Valenčáková-Agyagosová, Alexandra and Amrichová, Judita and Rosocha, Ján and Vaško, Gabriel and Švihla, Róbert and Petrovič, Vladimír", year = "2014", abstract = "The topic of the study was to verify in vivo survival of in vitro cultured autologous and allogenous chondrocytes suspended in a fi brin glue Beriplast® or seeded on Collagen type I-Hyaluronan (Col type I-HYA) scaffolds for the regeneration of articular cartilage defects in rabbits. The study was carried out on 15 domestic rabbits randomly assigned to fi ve groups (n = 3 in each) with different treatments of artifi cially created chondral defects (ChD ́s). These defects were made in a non-load-bearing area of medial condyle of the distal femur, and were treated as follows: 1st and 3rd group: the ChD ́s were fi lled with autologous or allogenous chondrocytes seeded on Col type I-HYA scaffolds, respectively. The scaffolds were fi xed to the ChD ́s by fi brin glue Beriplast®; 2nd and 4th group: the ChD ́s were fi lled with a suspension of autologous or allogenous chondrocytes in fi brin glue Beriplast®, respectively, and they were immediately covered by unseeded Col type I-HYA scaffolds; Control group: the ChD ́s were left to heal spontaneously without any treatment. Macroscopical, histological and immunohistochemical analyses of the ChD ́s were performed 12 months after the treatment. In all treated groups, the chondrocytes were capable to proliferate and produce the cartilage extracellular matrix, including proteoglycans and type II collagen, as compared to the control “untreated” group. On the other hand, the production of hyaline-like cartilage tissue confi rmed that both therapeutic methods using autologous chondrocytes can be applied successfully for the treatment of chondral defects in rabbits., Cilj studije je bio da se verifi kuje in vivo preživljavanje in vitro kultivisanih autolognih i alogenih hondrocita suspendovanih u fi brinski Beriplast® lepak ili na kolagen Hyaluronan tip I (Col type I-HYA) osnovu radi regeneracije oštećenja zglobne hrskavice kod kunića. Ispitivanje je obavljeno na 15 domaćih kunića, odabranih slučajnim izborom koji su podeljeni u pet grupa (3 po grupi) kod kojih su veštački izazvana oštećenja hrskavice (ChD), tretirana na različite načine. Oštećenja su napravljena na površinama koje nisu noseće na medijalnom kondilusu distalnog femura, a tretman je podrazumevao: prva i treća grupa: ChD su punjene sa autolognim ili alogenim hondrocitima zasejanim na Col tip I-HYA osnovu. Osnove su fi ksirane na ChD pomoću fi brinskog lepka Beriplast®; druga i četvrta grupa: oštećenja su punjena suspenzijom autolognih ili alogenih hondrocita u fi brinskom Beriplast® lepku, a neposredno posle toga, prekriveni su sa nezasejanom Col tip I-HYA osnovom; kontrolna grupa: oštećenja su ostavljena da spontano zarastu, bez tretmana. Dvanaest meseci posle tretmana obavljene su makroskopske, histološke i imunohistološke analize promena. Kod svih životinja tretiranih grupa, hondrociti su bili sposobni da proliferišu i proizvode ekstracelularni matriks hrskavice, uključujući proteoglikane i II tip kolagena u poređenju sa kontrolnom “netretriranom” grupom. Sa druge strane, proizvodnja hrskavičavog tkiva nalik na hijalin potvrđuje da se oba terapijska metoda koji koriste autologne hondrocite mogu da uspešno primene u tretiranju defekata hrskavice kod kunića.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue, Reparacija defekata hrskavice kunića autolognim i alogenim hondrocitima zasejanim na kolagen/ hijaluronan osnovu ili suspendovanim u fibrinskom lepku", volume = "64", number = "1", pages = "61-72", doi = "10.2478/acve-2014-0007" }
Horňák, S., Harvanová, D., Ledecký, V., Hluchý, M., Valenčáková-Agyagosová, A., Amrichová, J., Rosocha, J., Vaško, G., Švihla, R.,& Petrovič, V.. (2014). Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1), 61-72. https://doi.org/10.2478/acve-2014-0007
Horňák S, Harvanová D, Ledecký V, Hluchý M, Valenčáková-Agyagosová A, Amrichová J, Rosocha J, Vaško G, Švihla R, Petrovič V. Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue. in Acta Veterinaria-Beograd. 2014;64(1):61-72. doi:10.2478/acve-2014-0007 .
Horňák, Slavomír, Harvanová, Denisa, Ledecký, Valent, Hluchý, Marian, Valenčáková-Agyagosová, Alexandra, Amrichová, Judita, Rosocha, Ján, Vaško, Gabriel, Švihla, Róbert, Petrovič, Vladimír, "Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue" in Acta Veterinaria-Beograd, 64, no. 1 (2014):61-72, https://doi.org/10.2478/acve-2014-0007 . .