Приказ основних података о документу
Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
Karakteristike dijagnostičkih testova za dokazivanje antitela na virus reproduktivnog i respiratornog sindroma svinja u serumima sa farmi svinja u regionu sa velikom heterogenošću virusa
dc.creator | Karniychuk, Uladzimir U | |
dc.creator | Nauwynck, Hans J | |
dc.date.accessioned | 2021-08-20T07:04:42Z | |
dc.date.available | 2021-08-20T07:04:42Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/2140 | |
dc.description.abstract | AbstractPorcine reproductive and respiratory syndrome is the most economically important viral disease in the swine industry worldwide. Porcine reproductive and respiratory syndrome virus (PRRSV) strains are classified into two distinct genotypes, the European genotype and the North American genotype. The European PRRSV genotype has been divided into three subtypes: a pan-European subtype 1 and East European subtypes 2 and 3. The aim of this study was to evaluate the performance of commercial and homemade serological assays to test field sera from a geographical region with an extreme PRRSV heterogeneity. Belarus became the country of choice for sample collection because heterologous PRRSV strains of all known European subtypes circulate in this country. Sera from Belarusian swine farms were tested in immunoperoxidase monolayer assays based on pan-European subtype 1, East European subtype 3 and North American strains as antigens and commercial enzyme-linked immunosorbent assays (IDEXX and INGEZIM). The obtained results suggest that none of the serological tools for PRRSV diagnosis can guarantee a flawless detection of antibodies at the individual animal level. Considering heterogeneity of recently isolated European PRRSV strains the problem can be relevant in many countries. | |
dc.description.abstract | Globalno najznačajnije oboljenje u svinjarstvu jeste reproduktivni i respiratorni sindrom svinja (PRRS). Virus, izazivač PRRS-a obuhvata dva različita genotipa: evropski i severnoamerički. Evropski genotip PRRS virusa je podeljen u tri subtipa: panevropski subtip 1 i istočnoevropski subtipovi 2 i 3. Cilj ispitivanja je bio evaluacija karakteristika sa jedne strane komercijalnih i sa druge, seroloških testova koji su napravljeni u našoj laboratoriji, a u cilju testiranja seruma svinja iz različitih geografskih regiona sa ekstremno velikom heterogenošću PRRS virusa. S obzirom da u Belorusiji cirkulišu svi poznati evropski subtipovi PRRS virusa, ova je zamlja odabrana kao izvor uzoraka seruma svinja. Serumi poreklom sa farmi svinja iz Belorusije, ispitivani su metodom immunoperoksidaze u kulturi ćelija pri čemu su upotrebljeni antigeni: panevropskog subtip 1, istočnoevropskog subtip 3 i severnoameričkog soja uz primenu i komercijalne ELISA metode (IDEXX i INGEZIM). Dobijeni rezultati ukazuju da nijedna serološka metoda koja se koristi u dijagnostici PRRS virusa ne može da garantuje detekciju antitela na individualnom nivou. S obzirom na heterogenost nedavnih evropskih izolata PRRS virusnih sojeva, ovo može da bude značajno za veći broj zemalja. | |
dc.language | en | |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Acta Veterinaria-Beograd | |
dc.subject | porcine reproductive and respiratory syndrome virus | |
dc.subject | serology | |
dc.subject | diagnosis | |
dc.subject | Belarus | |
dc.title | Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity | |
dc.title | Karakteristike dijagnostičkih testova za dokazivanje antitela na virus reproduktivnog i respiratornog sindroma svinja u serumima sa farmi svinja u regionu sa velikom heterogenošću virusa | |
dc.type | article | en |
dc.rights.license | BY | |
dcterms.abstract | Науwyнцк, Ханс Ј; Карниyцхук, Уладзимир У; Карактеристике дијагностичких тестова за доказивање антитела на вирус репродуктивног и респираторног синдрома свиња у серумима са фарми свиња у региону са великом хетерогеношћу вируса; | |
dc.citation.volume | 64 | |
dc.citation.issue | 1 | |
dc.citation.spage | 44 | |
dc.citation.epage | 51 | |
dc.citation.rank | M23 | |
dc.identifier.doi | 10.2478/acve-2014-0005 | |
dc.identifier.scopus | 2-s2.0-84902507507 | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/5855/Karniychuk.pdf | |
dc.type.version | publishedVersion |